LOCUS       BBa_B3101               2867 bp    DNA     linear       22-Dec-2024
DEFINITION  I13506 B1101
ACCESSION   BBa_B3101
VERSION     BBa_B3101.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1956
                     /label=GFP protein
     CDS             1237..1956
                     /label=BBa_E0040
     misc_feature    1..1956
                     /label=BBa_I13506
     stem_loop       1974..1989
                     /label=stem loop
     polyA_site      1990..1998
                     /label=Poly A tail
     polyA_site      1965..1973
                     /label=PolyA tail
     misc_feature    1965..1998
                     /label=B1001
     terminator      1965..1998
                     /label=BBa_B1001
     misc_feature    2011..2014
                     /label=conserved
     RBS             2007..2018
                     /label=BBa_B0034
     CDS             2025..2699
                     /label=mrfp1
     misc_feature    2706..2730
                     /label=Help:Barcodes
     CDS             2025..2705
                     /label=BBa_E1010
     stem_loop       2750..2793
                     /label=stem_loop
     misc_feature    2739..2818
                     /label=BBa_B0010
     terminator      2739..2818
                     /label=BBa_B0010
     stem_loop       2834..2853
                     /label=T7 TE
     misc_feature    2860..2860
                     /label=stop
     polyA_site      2854..2867
                     /label=polya
     misc_feature    2827..2867
                     /label=BBa_B0012
     terminator      2827..2867
                     /label=BBa_B0012
     misc_feature    2007..2867
                     /label=BBa_I13507
     misc_feature    1965..2867
                     /label=BBa_B1101
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc
     1261 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     1321 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
     1381 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
     1441 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
     1501 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     1561 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     1621 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     1681 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
     1741 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     1801 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     1861 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     1921 attacacatg gcatggatga actatacaaa taataatact agagaaaaaa aaaccccgct
     1981 tcggcggggt ttttttttta ctagagaaag aggagaaata ctagatggct tcctccgaag
     2041 acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg
     2101 agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac
     2161 tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc
     2221 agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt
     2281 ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta
     2341 ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg
     2401 gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt
     2461 ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga
     2521 aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac
     2581 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
     2641 aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
     2701 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa
     2761 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     2821 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//