LOCUS BBa_B3107 2873 bp DNA linear 31-Oct-2024 DEFINITION I13506 B1107 ACCESSION BBa_B3107 VERSION BBa_B3107.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1956 /label=GFP protein CDS 1237..1956 /label=BBa_E0040 misc_feature 1..1956 /label=BBa_I13506 misc_feature 1965..2004 /label=B1007 stem_loop 1974..1995 /label=modified thr polyA_site 1968..1973 /label=PolyA polyA_site 1996..2001 /label=PolyA terminator 1965..2004 /label=BBa_B1007 misc_feature 2017..2020 /label=conserved RBS 2013..2024 /label=BBa_B0034 CDS 2031..2705 /label=mrfp1 misc_feature 2712..2736 /label=Help:Barcodes CDS 2031..2711 /label=BBa_E1010 stem_loop 2756..2799 /label=stem_loop misc_feature 2745..2824 /label=BBa_B0010 terminator 2745..2824 /label=BBa_B0010 stem_loop 2840..2859 /label=T7 TE misc_feature 2866..2866 /label=stop polyA_site 2860..2873 /label=polya misc_feature 2833..2873 /label=BBa_B0012 terminator 2833..2873 /label=BBa_B0012 misc_feature 2013..2873 /label=BBa_I13507 misc_feature 1965..2873 /label=BBa_B1107 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc 1261 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct 1321 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc 1381 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt 1441 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg 1501 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca 1561 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt 1621 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac 1681 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga 1741 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt 1801 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg 1861 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg 1921 attacacatg gcatggatga actatacaaa taataatact agagcgcaaa aaaccccgcc 1981 cctgacaggg cggggttttt tcgctactag agaaagagga gaaatactag atggcttcct 2041 ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg 2101 gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg 2161 ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc 2221 agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga 2281 aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg 2341 ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac 2401 tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg 2461 aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc 2521 gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta 2581 aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc 2641 acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg 2701 gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc atcaaataaa 2761 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 2821 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //