LOCUS       BBa_B3108               2873 bp    DNA     linear       10-Jun-2024
DEFINITION  I13506 B1108
ACCESSION   BBa_B3108
VERSION     BBa_B3108.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1956
                     /label=GFP protein
     CDS             1237..1956
                     /label=BBa_E0040
     misc_feature    1..1956
                     /label=BBa_I13506
     misc_feature    1965..2004
                     /label=B1008
     stem_loop       1974..1995
                     /label=modified thr terminator
     polyA_site      1969..1973
                     /label=PolyA
     polyA_site      1996..2000
                     /label=PolyA
     terminator      1965..2004
                     /label=BBa_B1008
     misc_feature    2017..2020
                     /label=conserved
     RBS             2013..2024
                     /label=BBa_B0034
     CDS             2031..2705
                     /label=mrfp1
     misc_feature    2712..2736
                     /label=Help:Barcodes
     CDS             2031..2711
                     /label=BBa_E1010
     stem_loop       2756..2799
                     /label=stem_loop
     misc_feature    2745..2824
                     /label=BBa_B0010
     terminator      2745..2824
                     /label=BBa_B0010
     stem_loop       2840..2859
                     /label=T7 TE
     misc_feature    2866..2866
                     /label=stop
     polyA_site      2860..2873
                     /label=polya
     misc_feature    2833..2873
                     /label=BBa_B0012
     terminator      2833..2873
                     /label=BBa_B0012
     misc_feature    2013..2873
                     /label=BBa_I13507
     misc_feature    1965..2873
                     /label=BBa_B1108
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc
     1261 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     1321 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
     1381 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
     1441 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
     1501 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     1561 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     1621 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     1681 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
     1741 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     1801 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     1861 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     1921 attacacatg gcatggatga actatacaaa taataatact agagcgccaa aaaccccgcc
     1981 cctgacaggg cggggttttt ccgctactag agaaagagga gaaatactag atggcttcct
     2041 ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg
     2101 gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg
     2161 ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc
     2221 agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga
     2281 aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg
     2341 ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac
     2401 tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg
     2461 aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc
     2521 gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta
     2581 aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc
     2641 acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg
     2701 gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc atcaaataaa
     2761 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     2821 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//