LOCUS       BBa_B3206               2872 bp    DNA     linear       22-Dec-2024
DEFINITION  I13516 B1206
ACCESSION   BBa_B3206
VERSION     BBa_B3206.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1911
                     /label=mrfp1
     misc_feature    1918..1942
                     /label=Help:Barcodes
     CDS             1237..1917
                     /label=BBa_E1010
     misc_feature    1..1942
                     /label=BBa_I13516
     misc_feature    1951..1989
                     /label=B1006
     stem_loop       1960..1981
                     /label=modified thr terminator
     polyA_site      1982..1989
                     /label=PolyA
     polyA_site      1951..1959
                     /label=PolyA
     terminator      1951..1989
                     /label=BBa_B1006
     misc_feature    2002..2005
                     /label=conserved
     RBS             1998..2009
                     /label=BBa_B0034
     CDS             2016..2735
                     /label=GFP protein
     CDS             2016..2735
                     /label=BBa_E0040
     stem_loop       2755..2798
                     /label=stem_loop
     misc_feature    2744..2823
                     /label=BBa_B0010
     terminator      2744..2823
                     /label=BBa_B0010
     stem_loop       2839..2858
                     /label=T7 TE
     misc_feature    2865..2865
                     /label=stop
     polyA_site      2859..2872
                     /label=polya
     misc_feature    2832..2872
                     /label=BBa_B0012
     terminator      2832..2872
                     /label=BBa_B0012
     misc_feature    1951..2872
                     /label=BBa_B1206
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc
     1261 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa
     1321 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt
     1381 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt
     1441 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg
     1501 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc
     1561 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac
     1621 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa
     1681 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa
     1741 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag
     1801 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
     1861 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc
     1921 tgatagtgct agtgtagatc gctactagag aaaaaaaaac cccgcccctg acagggcggg
     1981 gttttttttt actagagaaa gaggagaaat actagatgcg taaaggagaa gaacttttca
     2041 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg
     2101 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca
     2161 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc
     2221 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc
     2281 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac
     2341 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg
     2401 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca
     2461 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac
     2521 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg
     2581 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga
     2641 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga
     2701 ttacacatgg catggatgaa ctatacaaat aataatacta gagccaggca tcaaataaaa
     2761 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2821 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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