LOCUS       BBa_B3210               2873 bp    DNA     linear       22-Dec-2024
DEFINITION  BI13516 B1210
ACCESSION   BBa_B3210
VERSION     BBa_B3210.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1911
                     /label=mrfp1
     misc_feature    1918..1942
                     /label=Help:Barcodes
     CDS             1237..1917
                     /label=BBa_E1010
     misc_feature    1..1942
                     /label=BBa_I13516
     misc_feature    1951..1990
                     /label=B1010
     stem_loop       1960..1981
                     /label=modified thr terminator
     polyA_site      1957..1959
                     /label=PolyA
     polyA_site      1982..1984
                     /label=PolyA
     terminator      1951..1990
                     /label=BBa_B1010
     misc_feature    2003..2006
                     /label=conserved
     RBS             1999..2010
                     /label=BBa_B0034
     CDS             2017..2736
                     /label=GFP protein
     CDS             2017..2736
                     /label=BBa_E0040
     stem_loop       2756..2799
                     /label=stem_loop
     misc_feature    2745..2824
                     /label=BBa_B0010
     terminator      2745..2824
                     /label=BBa_B0010
     stem_loop       2840..2859
                     /label=T7 TE
     misc_feature    2866..2866
                     /label=stop
     polyA_site      2860..2873
                     /label=polya
     misc_feature    2833..2873
                     /label=BBa_B0012
     terminator      2833..2873
                     /label=BBa_B0012
     misc_feature    1951..2873
                     /label=BBa_B1210
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc
     1261 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa
     1321 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt
     1381 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt
     1441 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg
     1501 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc
     1561 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac
     1621 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa
     1681 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa
     1741 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag
     1801 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
     1861 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc
     1921 tgatagtgct agtgtagatc gctactagag cgccgcaaac cccgcccctg acagggcggg
     1981 gtttcgccgc tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc
     2041 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     2101 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
     2161 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
     2221 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
     2281 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     2341 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     2401 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     2461 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
     2521 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     2581 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     2641 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     2701 attacacatg gcatggatga actatacaaa taataatact agagccaggc atcaaataaa
     2761 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     2821 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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