BBa_C0070
1
rhII
autoinducer synthetase for N-butyryl-HSL (BHL) and HHL
2004-01-26T12:00:00Z
2015-08-31T04:07:23Z
P. aeruginosa PA3476
Released HQ 2013
Autoinducer synthesis protein that produces N-butyryl-HSL which binds to RhlR, obtained from Pseudomonas aeruginosa.
false
false
_1_
0
24
7
In stock
false
Editing Check (1/28/04)
(0) BB sites cleaned
(1) ATG
(2) TAATAA
(3) Blast checks out (i.e., it's RhlI)
(4) Codon changes OK (AA and tRNA usage)
(5) Chassis might have conflict (w/ native autoinducer system?)
(6) ssrA tag added
(7) barcode added
(8) Codon optimized for expression in enteric bacteria
<P>
2 silent point mutations have been included into the sequence at base 138 from A to G and at base 576 from G to C to remove internal EcoRI and PstI sites. Mutations were chosen to yield the same amino acid and codons of similar frequency in E. coli.
true
Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
annotation301203
1
rhlL
range301203
1
1
603
annotation2213996
1
Help:Barcodes
range2213996
1
643
667
annotation306820
1
LVA
range306820
1
604
636
BBa_C0070_sequence
1
atgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccaggttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgcttacctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtcccgttacgctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacctgggtgcttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgtctgggtccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaatgctgctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z