LOCUS BBa_C0480 1205 bp DNA linear 25-Nov-2024 DEFINITION Bgl Terminator with cfp reportor- expression system ACCESSION BBa_C0480 VERSION BBa_C0480.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 stem_loop 131..182 /label=bgl terminator terminator 64..187 /label=BBa_C0440 stem_loop 263..314 /label=bgl terminator terminator 196..319 /label=BBa_C0440 misc_feature 332..335 /label=conserved RBS 328..339 /label=BBa_B0034 CDS 346..1068 /label=BBa_E0020 stem_loop 1088..1131 /label=stem_loop misc_feature 1077..1156 /label=BBa_B0010 terminator 1077..1156 /label=BBa_B0010 stem_loop 1172..1191 /label=T7 TE misc_feature 1198..1198 /label=stop polyA_site 1192..1205 /label=polya misc_feature 1165..1205 /label=BBa_B0012 terminator 1165..1205 /label=BBa_B0012 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gaggaaatat tattactgag taaaggattg ttaccgcact aagcgggcaa aacctgaaaa 121 aaattgcttg attcacgtca ggccgttttt ttcaggtttt tttttggagt tttgccgcaa 181 agcggtatac tagaggaaat attattactg agtaaaggat tgttaccgca ctaagcgggc 241 aaaacctgaa aaaaattgct tgattcacgt caggccgttt ttttcaggtt tttttttgga 301 gttttgccgc aaagcggtat actagagaaa gaggagaaat actagatggt gagcaagggc 361 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtgaacggc 421 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 481 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 541 acctggggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc 601 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 661 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 721 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 781 tacatcagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac 841 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 901 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag 961 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 1021 accgccgccg ggatcactct cggcatggac gagctgtaca agtaataata ctagagccag 1081 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 1141 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 1201 ttata //