LOCUS       BBa_C0500               1205 bp    DNA     linear       22-Dec-2024
DEFINITION  Bgl Terminator with yfp reportor- expression system
ACCESSION   BBa_C0500
VERSION     BBa_C0500.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     stem_loop       131..182
                     /label=bgl terminator
     terminator      64..187
                     /label=BBa_C0440
     stem_loop       263..314
                     /label=bgl terminator
     terminator      196..319
                     /label=BBa_C0440
     terminator      64..319
                     /label=BBa_C0450
     misc_feature    332..335
                     /label=conserved
     RBS             328..339
                     /label=BBa_B0034
     CDS             346..1068
                     /label=BBa_E0030
     stem_loop       1088..1131
                     /label=stem_loop
     misc_feature    1077..1156
                     /label=BBa_B0010
     terminator      1077..1156
                     /label=BBa_B0010
     stem_loop       1172..1191
                     /label=T7 TE
     misc_feature    1198..1198
                     /label=stop
     polyA_site      1192..1205
                     /label=polya
     misc_feature    1165..1205
                     /label=BBa_B0012
     terminator      1165..1205
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gaggaaatat tattactgag taaaggattg ttaccgcact aagcgggcaa aacctgaaaa
      121 aaattgcttg attcacgtca ggccgttttt ttcaggtttt tttttggagt tttgccgcaa
      181 agcggtatac tagaggaaat attattactg agtaaaggat tgttaccgca ctaagcgggc
      241 aaaacctgaa aaaaattgct tgattcacgt caggccgttt ttttcaggtt tttttttgga
      301 gttttgccgc aaagcggtat actagagaaa gaggagaaat actagatggt gagcaagggc
      361 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
      421 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      481 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
      541 ggctacggcc tgcaatgctt cgcccgctac cccgaccaca tgaagctgca cgacttcttc
      601 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      661 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      721 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      781 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
      841 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
      901 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
      961 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1021 accgccgccg ggatcactct cggcatggac gagctgtaca agtaataata ctagagccag
     1081 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1141 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1201 ttata
//