LOCUS       BBa_C0510               2418 bp    DNA     linear       14-May-2024
DEFINITION  Combined expession system of cfp and yfp downstream to bgl 
            antiterminators
ACCESSION   BBa_C0510
VERSION     BBa_C0510.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     stem_loop       131..182
                     /label=bgl terminator
     terminator      64..187
                     /label=BBa_C0440
     stem_loop       263..314
                     /label=bgl terminator
     terminator      196..319
                     /label=BBa_C0440
     misc_feature    332..335
                     /label=conserved
     RBS             328..339
                     /label=BBa_B0034
     CDS             346..1068
                     /label=BBa_E0020
     stem_loop       1088..1131
                     /label=stem_loop
     misc_feature    1077..1156
                     /label=BBa_B0010
     terminator      1077..1156
                     /label=BBa_B0010
     stem_loop       1172..1191
                     /label=T7 TE
     misc_feature    1198..1198
                     /label=stop
     polyA_site      1192..1205
                     /label=polya
     misc_feature    1165..1205
                     /label=BBa_B0012
     terminator      1165..1205
                     /label=BBa_B0012
     misc_feature    1216..1232
                     /label=lac O1
     promoter        1233..1238
                     /label=-35
     misc_feature    1239..1255
                     /label=lac O1
     promoter        1256..1261
                     /label=-10
     misc_feature    1214..1267
                     /label=BBa_R0011
     promoter        1214..1267
                     /label=BBa_R0011
     stem_loop       1344..1395
                     /label=bgl terminator
     terminator      1277..1400
                     /label=BBa_C0440
     stem_loop       1476..1527
                     /label=bgl terminator
     terminator      1409..1532
                     /label=BBa_C0440
     terminator      1277..1532
                     /label=BBa_C0450
     misc_feature    1545..1548
                     /label=conserved
     RBS             1541..1552
                     /label=BBa_B0034
     CDS             1559..2281
                     /label=BBa_E0030
     stem_loop       2301..2344
                     /label=stem_loop
     misc_feature    2290..2369
                     /label=BBa_B0010
     terminator      2290..2369
                     /label=BBa_B0010
     stem_loop       2385..2404
                     /label=T7 TE
     misc_feature    2411..2411
                     /label=stop
     polyA_site      2405..2418
                     /label=polya
     misc_feature    2378..2418
                     /label=BBa_B0012
     terminator      2378..2418
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gaggaaatat tattactgag taaaggattg ttaccgcact aagcgggcaa aacctgaaaa
      121 aaattgcttg attcacgtca ggccgttttt ttcaggtttt tttttggagt tttgccgcaa
      181 agcggtatac tagaggaaat attattactg agtaaaggat tgttaccgca ctaagcgggc
      241 aaaacctgaa aaaaattgct tgattcacgt caggccgttt ttttcaggtt tttttttgga
      301 gttttgccgc aaagcggtat actagagaaa gaggagaaat actagatggt gagcaagggc
      361 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtgaacggc
      421 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
      481 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
      541 acctggggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
      601 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
      661 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
      721 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
      781 tacatcagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
      841 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
      901 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
      961 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1021 accgccgccg ggatcactct cggcatggac gagctgtaca agtaataata ctagagccag
     1081 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1141 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1201 ttatatacta gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac
     1261 tgagcacata ctagaggaaa tattattact gagtaaagga ttgttaccgc actaagcggg
     1321 caaaacctga aaaaaattgc ttgattcacg tcaggccgtt tttttcaggt ttttttttgg
     1381 agttttgccg caaagcggta tactagagga aatattatta ctgagtaaag gattgttacc
     1441 gcactaagcg ggcaaaacct gaaaaaaatt gcttgattca cgtcaggccg tttttttcag
     1501 gttttttttt ggagttttgc cgcaaagcgg tatactagag aaagaggaga aatactagat
     1561 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1621 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1681 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1741 cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct
     1801 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1861 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1921 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1981 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2041 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2101 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2161 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2221 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaata
     2281 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2341 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2401 ggcctttctg cgtttata
//