BBa_C0510
type: ComponentDefinition
persistent identity: BBa C0510
display id: BBa_C0510
version: 1
was generated by: iGEM to SBOL conversion
description: Combined expession system of cfp and yfp downstream to bgl antiterminators
created: 2006-03-30T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
group u list: _44_
m user id: 0
owner id: 439
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: T. Senthil
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_E0020
component: BBa_B0034
component: BBa_E0030
component: BBa_C0450
component: BBa_R0011
component: BBa_C0440
sequence annotation: BBa_R0011
sequence annotation: BBa_C0440
sequence annotation: BBa_B0034
sequence annotation: BBa_E0020
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_C0450
sequence annotation: BBa_E0030
sequence: https://synbiohub.org/public/igem/BBa_C0510_sequence/1

BBa_B0012
type: Component
persistent identity: component1830389
display id: component1830389
version: 1
top level: BBa_C0510
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1830379
display id: component1830379
version: 1
top level: BBa_C0510
definition: BBa_B0010
access: public

BBa_B0012
type: Component
persistent identity: component1830335
display id: component1830335
version: 1
top level: BBa_C0510
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1830325
display id: component1830325
version: 1
top level: BBa_C0510
definition: BBa_B0010
access: public

BBa_E0020
type: Component
persistent identity: component1830320
display id: component1830320
version: 1
top level: BBa_C0510
definition: ecfp
access: public

BBa_B0034
type: Component
persistent identity: component1830318
display id: component1830318
version: 1
top level: BBa_C0510
definition: BBa_B0034
access: public

BBa_E0030
type: Component
persistent identity: component1830374
display id: component1830374
version: 1
top level: BBa_C0510
definition: eyfp
access: public

BBa_B0034
type: Component
persistent identity: component1830372
display id: component1830372
version: 1
top level: BBa_C0510
definition: BBa_B0034
access: public

BBa_C0450
type: Component
persistent identity: component1830367
display id: component1830367
version: 1
top level: BBa_C0510
definition: BBa_C0450
access: public

BBa_R0011
type: Component
persistent identity: component1830349
display id: component1830349
version: 1
top level: BBa_C0510
definition: lacI+pL
access: public

BBa_C0440
type: Component
persistent identity: component1830313
display id: component1830313
version: 1
top level: BBa_C0510
definition: BBa_C0440
access: public

BBa_C0440
type: Component
persistent identity: component1830308
display id: component1830308
version: 1
top level: BBa_C0510
definition: BBa_C0440
access: public

BBa_R0011
type: Component
persistent identity: component1830299
display id: component1830299
version: 1
top level: BBa_C0510
definition: lacI+pL
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1830299
display id: annotation1830299
version: 1
top level: BBa_C0510
location: https://synbiohub.org/public/igem/BBa_C0510/annotation1830299/range1830299/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_C0510/annotation1830299/range1830299/1
type: Range
persistent identity: range1830299
display id: range1830299
version: 1
top level: BBa_C0510
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_C0440
type: SequenceAnnotation
persistent identity: annotation1830308
display id: annotation1830308
version: 1
top level: BBa_C0510
location: https://synbiohub.org/public/igem/BBa_C0510/annotation1830308/range1830308/1
component: BBa_C0440

https://synbiohub.org/public/igem/BBa_C0510/annotation1830308/range1830308/1
type: Range
persistent identity: range1830308
display id: range1830308
version: 1
top level: BBa_C0510
direction: direction/reverse
start: 64
end: 187
orientation: inline

BBa_C0440
type: SequenceAnnotation
persistent identity: annotation1830313
display id: annotation1830313
version: 1
top level: BBa_C0510
location: https://synbiohub.org/public/igem/BBa_C0510/annotation1830313/range1830313/1
component: BBa_C0440

https://synbiohub.org/public/igem/BBa_C0510/annotation1830313/range1830313/1
type: Range
persistent identity: range1830313
display id: range1830313
version: 1
top level: BBa_C0510
direction: direction/reverse
start: 196
end: 319
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1830318
display id: annotation1830318
version: 1
top level: BBa_C0510
location: https://synbiohub.org/public/igem/BBa_C0510/annotation1830318/range1830318/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_C0510/annotation1830318/range1830318/1
type: Range
persistent identity: range1830318
display id: range1830318
version: 1
top level: BBa_C0510
direction: direction/reverse
start: 328
end: 339
orientation: inline

BBa_E0020
type: SequenceAnnotation
persistent identity: annotation1830320
display id: annotation1830320
version: 1
top level: BBa_C0510
location: https://synbiohub.org/public/igem/BBa_C0510/annotation1830320/range1830320/1
component: BBa_E0020

https://synbiohub.org/public/igem/BBa_C0510/annotation1830320/range1830320/1
type: Range
persistent identity: range1830320
display id: range1830320
version: 1
top level: BBa_C0510
direction: direction/reverse
start: 346
end: 1068
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1830325
display id: annotation1830325
version: 1
top level: BBa_C0510
location: https://synbiohub.org/public/igem/BBa_C0510/annotation1830325/range1830325/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_C0510/annotation1830325/range1830325/1
type: Range
persistent identity: range1830325
display id: range1830325
version: 1
top level: BBa_C0510
direction: direction/forward
start: 1077
end: 1156
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1830335
display id: annotation1830335
version: 1
top level: BBa_C0510
location: https://synbiohub.org/public/igem/BBa_C0510/annotation1830335/range1830335/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_C0510/annotation1830335/range1830335/1
type: Range
persistent identity: range1830335
display id: range1830335
version: 1
top level: BBa_C0510
direction: direction/forward
start: 1165
end: 1205
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1830349
display id: annotation1830349
version: 1
top level: BBa_C0510
location: https://synbiohub.org/public/igem/BBa_C0510/annotation1830349/range1830349/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_C0510/annotation1830349/range1830349/1
type: Range
persistent identity: range1830349
display id: range1830349
version: 1
top level: BBa_C0510
direction: direction/forward
start: 1214
end: 1267
orientation: inline

BBa_C0450
type: SequenceAnnotation
persistent identity: annotation1830367
display id: annotation1830367
version: 1
top level: BBa_C0510
location: https://synbiohub.org/public/igem/BBa_C0510/annotation1830367/range1830367/1
component: BBa_C0450

https://synbiohub.org/public/igem/BBa_C0510/annotation1830367/range1830367/1
type: Range
persistent identity: range1830367
display id: range1830367
version: 1
top level: BBa_C0510
direction: direction/reverse
start: 1277
end: 1532
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1830372
display id: annotation1830372
version: 1
top level: BBa_C0510
location: https://synbiohub.org/public/igem/BBa_C0510/annotation1830372/range1830372/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_C0510/annotation1830372/range1830372/1
type: Range
persistent identity: range1830372
display id: range1830372
version: 1
top level: BBa_C0510
direction: direction/reverse
start: 1541
end: 1552
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1830374
display id: annotation1830374
version: 1
top level: BBa_C0510
location: https://synbiohub.org/public/igem/BBa_C0510/annotation1830374/range1830374/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_C0510/annotation1830374/range1830374/1
type: Range
persistent identity: range1830374
display id: range1830374
version: 1
top level: BBa_C0510
direction: direction/reverse
start: 1559
end: 2281
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1830379
display id: annotation1830379
version: 1
top level: BBa_C0510
location: https://synbiohub.org/public/igem/BBa_C0510/annotation1830379/range1830379/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_C0510/annotation1830379/range1830379/1
type: Range
persistent identity: range1830379
display id: range1830379
version: 1
top level: BBa_C0510
direction: direction/forward
start: 2290
end: 2369
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1830389
display id: annotation1830389
version: 1
top level: BBa_C0510
location: https://synbiohub.org/public/igem/BBa_C0510/annotation1830389/range1830389/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_C0510/annotation1830389/range1830389/1
type: Range
persistent identity: range1830389
display id: range1830389
version: 1
top level: BBa_C0510
direction: direction/forward
start: 2378
end: 2418
orientation: inline

BBa_C0440
type: ComponentDefinition
persistent identity: BBa C0440
display id: BBa_C0440
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (bgl)
created: 2005-10-28T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
mutable description: Terminator in Bgl operon that is antiterminated by bglG
discontinued: false
dominant: false
group u list: _44_
m user id: 0
owner id: 439
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: T. Senthil
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: bgl terminator
sequence: https://synbiohub.org/public/igem/BBa_C0440_sequence/1

bgl terminator
type: SequenceAnnotation
persistent identity: annotation1744294
display id: annotation1744294
version: 1
top level: BBa_C0440
location: https://synbiohub.org/public/igem/BBa_C0440/annotation1744294/range1744294/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_C0440/annotation1744294/range1744294/1
type: Range
persistent identity: range1744294
display id: range1744294
version: 1
top level: BBa_C0440
direction: direction/forward
start: 68
end: 119
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

ecfp
type: ComponentDefinition
persistent identity: BBa E0020
display id: BBa_E0020
version: 1
was generated by: iGEM to SBOL conversion
description: engineered cyan fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_E0020_sequence/1

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_C0450
type: ComponentDefinition
persistent identity: BBa C0450
display id: BBa_C0450
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (C0440 + C0440)
created: 2005-10-28T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
mutable description: Two terminators in Bgl operon that is antiterminated by bglG
discontinued: false
dominant: false
group u list: _44_
m user id: 0
owner id: 439
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: T. Senthil
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_C0440
sequence annotation: BBa_C0440
sequence: https://synbiohub.org/public/igem/BBa_C0450_sequence/1

BBa_C0440
type: Component
persistent identity: component1744577
display id: component1744577
version: 1
top level: BBa_C0450
definition: BBa_C0440
access: public

BBa_C0440
type: Component
persistent identity: component1744572
display id: component1744572
version: 1
top level: BBa_C0450
definition: BBa_C0440
access: public

BBa_C0440
type: SequenceAnnotation
persistent identity: annotation1744572
display id: annotation1744572
version: 1
top level: BBa_C0450
location: https://synbiohub.org/public/igem/BBa_C0450/annotation1744572/range1744572/1
component: BBa_C0440

https://synbiohub.org/public/igem/BBa_C0450/annotation1744572/range1744572/1
type: Range
persistent identity: range1744572
display id: range1744572
version: 1
top level: BBa_C0450
direction: direction/forward
start: 1
end: 124
orientation: inline

BBa_C0440
type: SequenceAnnotation
persistent identity: annotation1744577
display id: annotation1744577
version: 1
top level: BBa_C0450
location: https://synbiohub.org/public/igem/BBa_C0450/annotation1744577/range1744577/1
component: BBa_C0440

https://synbiohub.org/public/igem/BBa_C0450/annotation1744577/range1744577/1
type: Range
persistent identity: range1744577
display id: range1744577
version: 1
top level: BBa_C0450
direction: direction/forward
start: 133
end: 256
orientation: inline

eyfp
type: ComponentDefinition
persistent identity: BBa E0030
display id: BBa_E0030
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modificaitons to Clontech EYFP by Reshma Shetty
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_E0030_sequence/1

https://synbiohub.org/public/igem/BBa_C0510_sequence/1
type: Sequence
persistent identity: BBa C0510 sequence
display id: BBa_C0510_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagaggaaatattattactgagtaaaggattgttaccgcactaagcgggcaaaacctgaaaaaaattgcttgattcacgtcaggccgtttttttcaggtttttttttggagttttgccgcaaagcggtatactagaggaaatattattactgagtaaaggattgttaccgcactaagcgggcaaaacctgaaaaaaattgcttgattcacgtcaggccgtttttttcaggtttttttttggagttttgccgcaaagcggtatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagaggaaatattattactgagtaaaggattgttaccgcactaagcgggcaaaacctgaaaaaaattgcttgattcacgtcaggccgtttttttcaggtttttttttggagttttgccgcaaagcggtatactagaggaaatattattactgagtaaaggattgttaccgcactaagcgggcaaaacctgaaaaaaattgcttgattcacgtcaggccgtttttttcaggtttttttttggagttttgccgcaaagcggtatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaaca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0440_sequence/1
type: Sequence
persistent identity: BBa C0440 sequence
display id: BBa_C0440_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaatattattactgagtaaaggattgttaccgcactaagcgggcaaaacctgaaaaaaattgcttgattcacgtcaggccgtttttttcaggtttttttttggagttttgccgcaaagcggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0020_sequence/1
type: Sequence
persistent identity: BBa E0020 sequence
display id: BBa_E0020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0450_sequence/1
type: Sequence
persistent identity: BBa C0450 sequence
display id: BBa_C0450_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaatattattactgagtaaaggattgttaccgcactaagcgggcaaaacctgaaaaaaattgcttgattcacgtcaggccgtttttttcaggtttttttttggagttttgccgcaaagcggtatactagaggaaatattattactgagtaaaggattgttaccgcactaagcgggcaaaacctgaaaaaaattgcttgattcacgtcaggccgtttttttcaggtttttttttggagttttgccgcaaagcggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0030_sequence/1
type: Sequence
persistent identity: BBa E0030 sequence
display id: BBa_E0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z