BBa_C2000
1
zif23
Repressor, Zif23-GCN4 (RBS-, LVA+)
2004-01-25T12:00:00Z
2015-08-31T04:07:25Z
Zif268 and GCN4 [Wolfe00]
Coding region for Zif23-GCN4. This protein is constructed out of fingers 2 and 3 from Zif268 fused with the leucine zipper domain from GCN4. The protein binds cooperatively as a dimer to a symmetric target DNA sequence in which each monomer targets 5'-GCGTGG-3'.
false
false
_1_
0
24
7
Not in stock
false
As in [Wolfe00], the protein uses residues 364-412 of Zif268:
<a href="http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=BAC30748.1">BAC30748.1</a>
<br>
The corresponding nucleotide sequence was taken from:
<a href="http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=AK040925.1">AK040925.1</a>
<br>
<P>As in [Wolfe00], the protein uses residues 250-281 of GCN4:
<a href="http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=AAA34640.1">AAA34640.1</a>
<br>
The corresponding nucleotide sequence was taken from:
<a href="http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=171581">K02205.1</a>
<br>
false
Ronny Krashinsky
annotation2213994
1
Help:Barcodes
range2213994
1
268
292
annotation297319
1
Zif268: Finger 2
range297319
1
7
90
annotation297320
1
Zif268: Finger 3 (partial)
range297320
1
91
150
annotation297322
1
GCN4: leucine zipper
range297322
1
166
261
annotation297321
1
linker
range297321
1
151
165
annotation297323
1
Finger2 Recognition
range297323
1
43
63
annotation297324
1
Finger 3 Recognition
range297324
1
127
147
BBa_C2000_sequence
1
atgaagcccttccagtgtcgaatctgcatgcgtaacttcagtcgtagtgaccaccttaccacccacatccgcacccacacaggcgagaagccttttgcctgtgacatttgtgggaggaagtttgccaggagtgatgaacgcaagaggcatagaaagttgcaacatatgaaacaacttgaagacaaggttgaagaattgctttcgaaaaattatcacttggaaaatgaggttgccagattaaagaaattagttggcgaacgctaataacgctgatagtgctagtgtagatcgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z