BBa_C2008 1 BBa_C2008 MBP.p53ZF F2F3.GCN4 homodimeric repressor 2007-08-21T11:00:00Z 2015-08-31T04:07:25Z This coding region was rationally designed based on BBa_C2006 and p53ZF. Maltose binding protein fused to fingers 2 and 3 of p53ZF (a mutated Zif268 designed to bind to a different operator) fused to the GCN4 leucine zipper. false false _41_ 0 126 162 Not in stock false Two silent mutations were introduced to remove an internal NotI site and a rare arginine codon. false Reshma Shetty annotation1942324 1 GCN4 leucine zipper range1942324 1 1327 1422 annotation1942325 1 double TAA stop codon range1942325 1 1423 1428 annotation1942326 1 p53ZF F2F3 zinc finger DNA binding domain range1942326 1 1168 1311 annotation1942321 1 malE maltose binding protein (MBP) range1942321 1 1 1101 annotation1942323 1 MBP.p53ZF F2F3.GCN4 range1942323 1 1 1428 annotation1942322 1 start codon range1942322 1 1 3 BBa_C2008_sequence 1 atgaaaatcgaagaaggtaaactggtaatctggattaacggcgataaaggctataacggtctcgctgaagtcggtaagaaattcgagaaagataccggaattaaagtcaccgttgagcatccggataaactggaagagaaattcccacaggttgcggcaactggcgatggccctgacattatcttctgggcacacgaccgctttggtggctacgctcaatctggcctgttggctgaaatcaccccggacaaagcgttccaggacaagctgtatccgtttacctgggatgccgtacgttacaacggcaagctgattgcttacccgatcgctgttgaagcgttatcgctgatttataacaaagatctgctgccgaacccgccaaaaacctgggaagagatcccggcgctggataaagaactgaaagcgaaaggtaagagcgcgctgatgttcaacctgcaagaaccgtacttcacctggccgctgattgctgctgacgggggttatgcgttcaagtatgaaaacggcaagtacgacattaaagacgtgggcgtggataacgctggcgcgaaagcgggtctgaccttcctggttgacctgattaaaaacaaacacatgaatgcagacaccgattactccatcgcagaagctgcctttaataaaggcgaaacagcgatgaccatcaacggcccgtgggcatggtccaacatcgacaccagcaaagtgaattatggtgtaacggtactgccgaccttcaagggtcaaccatccaaaccgttcgttggcgtgctgagcgcaggtattaacgccgccagtccgaacaaagagctggcaaaagagttcctcgaaaactatctgctgactgatgaaggtctggaagcggttaataaagacaaaccgctgggtgccgtagcgctgaagtcttacgaggaagagttggcgaaagatccacgtattgccgccactatggaaaacgcccagaaaggtgaaatcatgccgaacatcccgcagatgtccgctttctggtatgccgtgcgtactgcggtgatcaacgccgccagcggtcgtcagactgtcgatgaagccctgaaagacgcgcagactaattcgagctcgaacaacaacaacaataacaataacaacaacctcgggatcgagggacggatgaagccgtttcaatgccgcatttgtatgcggaatttctcacagcgcggcacccttacccgccatattcgcacacacaccggggagaagccttttgcctgtgacatctgtggccgcaagttcgcgcgcctccaccaccgtctgctccatcgtaaattgcagcacatgaaacagctggaagacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaacgttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z