##gff-version 3
##sequence-region BBa_C20091 1 1430
BBa_C20091	.	start_codon	1	3	.	+	0	ID=annotation1963296;Name=Start codon
BBa_C20091	.	engineered_region	1	1430	.	+	0	ID=annotation1963297;Name=BBa_C20091
BBa_C20091	.	sequence_alteration	1188	1188	.	+	0	ID=annotation1963293;Name=mutation to A
BBa_C20091	.	CDS	1	1221	.	+	0	ID=annotation1963295;Name=Coding region
BBa_C20091	.	CDS	1	1101	.	+	0	ID=annotation1963283;Name=malE
BBa_C20091	.	sequence_alteration	1181	1181	.	+	0	ID=annotation1963292;Name=C insertion
BBa_C20091	.	stop_codon	1219	1221	.	+	0	ID=annotation1963294;Name=Premature TGA stop codon
>BBa_C20091
atgaaaatcgaagaaggtaaactggtaatctggattaacggcgataaaggctataacggtctcgctgaagtcggtaaga
aattcgagaaagataccggaattaaagtcaccgttgagcatccggataaactggaagagaaattcccacaggttgcggc
aactggcgatggccctgacattatcttctgggcacacgaccgctttggtggctacgctcaatctggcctgttggctgaa
atcaccccggacaaagcgttccaggacaagctgtatccgtttacctgggatgccgtacgttacaacggcaagctgattg
cttacccgatcgctgttgaagcgttatcgctgatttataacaaagatctgctgccgaacccgccaaaaacctgggaaga
gatcccggcgctggataaagaactgaaagcgaaaggtaagagcgcgctgatgttcaacctgcaagaaccgtacttcacc
tggccgctgattgctgctgacgggggttatgcgttcaagtatgaaaacggcaagtacgacattaaagacgtgggcgtgg
ataacgctggcgcgaaagcgggtctgaccttcctggttgacctgattaaaaacaaacacatgaatgcagacaccgatta
ctccatcgcagaagctgcctttaataaaggcgaaacagcgatgaccatcaacggcccgtgggcatggtccaacatcgac
accagcaaagtgaattatggtgtaacggtactgccgaccttcaagggtcaaccatccaaaccgttcgttggcgtgctga
gcgcaggtattaacgccgccagtccgaacaaagagctggcaaaagagttcctcgaaaactatctgctgactgatgaagg
tctggaagcggttaataaagacaaaccgctgggtgccgtagcgctgaagtcttacgaggaagagttggcgaaagatcca
cgtattgccgccactatggaaaacgcccagaaaggtgaaatcatgccgaacatcccgcagatgtccgctttctggtatg
ccgtgcgtactgcggtgatcaacgccgccagcggtcgtcagactgtcgatgaagccctgaaagacgcgcagactaattc
gagctcgaacaacaacaacaataacaataacaacaacctcgggatcgagggaaggatgaaaccgttccagtgcccgtat
ctagcatgcgtaacttctcccgttccgaccacctgaccacccacatccgtacccacaccggtgaaaaaccgttcgcttg
cgacatctgcggtcgtaaattcgctcgttccgacgaacgtaaacgtcaccgtaaattgcagcacatgaaacagctggaa
gacaaagttgaagaactgctgtccaaaaactaccacctggaaaacgaagttgctcgtctgaaaaaactggttggtgaac
gttaataa