BBa_E0050 1 BBa_E0050 GFP.lacZalpha fusion 2007-07-22T11:00:00Z 2015-08-31T04:07:25Z The GFP sequence comes from (BBa_E0040) and the N-terminal alpha fragment of lacZ comes directly from lacZ. This part is a fusion protein between GFP (BBa_E0040) and the N-terminal alpha fragment of lacZ (which encodes β-galactosidase. It is a reporter which can be measured via fluorescence from GFP or through a Miller assay of β-galactosidase. false false _41_ 0 126 162 Not in stock false I have included the complete GFP sequence. The boundaries of the lacZ alpha fragment are the first 60 codons of lacZ. This is what is defined as the alpha peptide in "The lac operon" by Benno Muller-Hill (see page 76). false Austin Che and Reshma Shetty annotation1939246 1 double stop codon range1939246 1 895 900 annotation1939244 1 BBa_E0040 (GFP) range1939244 1 1 714 annotation1939245 1 lacZ alpha peptide range1939245 1 715 894 BBa_E0050_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z