LOCUS       BBa_E0433                508 bp    DNA     linear       29-Apr-2024
DEFINITION  B0034.E0033.B0015
ACCESSION   BBa_E0433
VERSION     BBa_E0433.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..366
                     /label=lacZ alpha
     misc_feature    99..99
                     /label=T
     misc_feature    105..105
                     /label=A
     misc_feature    126..126
                     /label=C
     misc_feature    96..96
                     /label=C
     misc_feature    129..129
                     /label=G
     promoter        44..53
                     /label=T3 promoter
     promoter        191..209
                     /label=T7 promoter
     misc_feature    217..366
                     /label=lacZ gene fragment
     misc_feature    19..33
                     /label=lacZ gene fragment
     misc_feature    19..371
                     /label=BBa_E0033
     stem_loop       391..434
                     /label=stem_loop
     misc_feature    380..459
                     /label=BBa_B0010
     terminator      380..459
                     /label=BBa_B0010
     stem_loop       475..494
                     /label=T7 TE
     misc_feature    501..501
                     /label=stop
     polyA_site      495..508
                     /label=polya
     misc_feature    468..508
                     /label=BBa_B0012
     terminator      468..508
                     /label=BBa_B0012
     terminator      380..508
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
       61 gggaacaaaa gctggagctc caccgcggtg gcggcagcac tagagctagt ggatcccccg
      121 ggctgtagaa attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta
      181 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
      241 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      301 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      361 ctgaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      421 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
      481 ccttcgggtg ggcctttctg cgtttata
//