LOCUS       BBa_E0435                534 bp    DNA     linear       30-Apr-2024
DEFINITION  PoPS->lacZalpha reporter device
ACCESSION   BBa_E0435
VERSION     BBa_E0435.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..33
                     /label=lacZ gene fragment
     CDS             19..372
                     /label=lacZ alpha
     promoter        44..53
                     /label=T3 promoter
     promoter        191..209
                     /label=T7 promoter
     misc_feature    96..96
                     /label=C
     misc_feature    99..99
                     /label=T
     misc_feature    105..105
                     /label=A
     misc_feature    126..126
                     /label=C
     misc_feature    129..129
                     /label=G
     misc_feature    217..366
                     /label=lacZ gene fragment
     misc_feature    366..366
                     /label=inserted T
     misc_feature    373..397
                     /label=barcode
     misc_feature    19..397
                     /label=BBa_E0035
     stem_loop       417..460
                     /label=stem_loop
     misc_feature    406..485
                     /label=BBa_B0010
     terminator      406..485
                     /label=BBa_B0010
     stem_loop       501..520
                     /label=T7 TE
     misc_feature    527..527
                     /label=stop
     polyA_site      521..534
                     /label=polya
     misc_feature    494..534
                     /label=BBa_B0012
     terminator      494..534
                     /label=BBa_B0012
     terminator      406..534
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
       61 gggaacaaaa gctggagctc caccgcggtg gcggcagcac tagagctagt ggatcccccg
      121 ggctgtagaa attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta
      181 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
      241 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      301 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      361 ctgaattaat aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa
      421 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      481 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//