##gff-version 3
##sequence-region BBa_E0435 1 534
BBa_E0435	.	engineered_region	19	397	.	+	0	ID=BBa_E0035;Name=BBa_E0035
BBa_E0435	.	sequence_alteration	126	126	.	+	0	ID=annotation1959042;Name=C;Parent=BBa_E0035
BBa_E0435	.	sequence_alteration	96	96	.	+	0	ID=annotation1959039;Name=C;Parent=BBa_E0035
BBa_E0435	.	sequence_alteration	99	99	.	+	0	ID=annotation1959040;Name=T;Parent=BBa_E0035
BBa_E0435	.	engineered_tag	373	397	.	+	0	ID=annotation1959046;Name=barcode;Parent=BBa_E0035
BBa_E0435	.	sequence_feature	19	33	.	+	0	ID=annotation1959035;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_E0435	.	promoter	44	53	.	+	0	ID=annotation1959037;Name=T3 promoter;Parent=BBa_E0035
BBa_E0435	.	sequence_alteration	366	366	.	+	0	ID=annotation1959045;Name=inserted T;Parent=BBa_E0035
BBa_E0435	.	CDS	19	372	.	+	0	ID=annotation1959036;Name=lacZ alpha;Parent=BBa_E0035
BBa_E0435	.	sequence_alteration	129	129	.	+	0	ID=annotation1959043;Name=G;Parent=BBa_E0035
BBa_E0435	.	sequence_feature	217	366	.	+	0	ID=annotation1959044;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_E0435	.	promoter	191	209	.	+	0	ID=annotation1959038;Name=T7 promoter;Parent=BBa_E0035
BBa_E0435	.	sequence_alteration	105	105	.	+	0	ID=annotation1959041;Name=A;Parent=BBa_E0035
BBa_E0435	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_E0435	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_E0435	.	terminator	406	534	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_E0435	.	terminator	494	534	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_E0435	.	polyA_site	521	534	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_E0435	.	engineered_region	494	534	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_E0435	.	stem_loop	501	520	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_E0435	.	stop_codon	527	527	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_E0435	.	terminator	406	485	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_E0435	.	engineered_region	406	485	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_E0435	.	stem_loop	417	460	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_E0435
aaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagct
ccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtc
gacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaa
cgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaata
gcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatc
gctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata