LOCUS BBa_E0600 3514 bp DNA linear 15-Nov-2024 DEFINITION I0500.Q04121.E0432 ACCESSION BBa_E0600 VERSION BBa_E0600.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 misc_feature 2326..2364 /label=LVA CDS 1237..2364 /label=lacI-LVA misc_feature 1237..2364 /label=BBa_C0012 misc_feature 2365..2389 /label=Help:Barcodes CDS 1237..2364 /label=BBa_C0012 stem_loop 2409..2452 /label=stem_loop misc_feature 2398..2477 /label=BBa_B0010 terminator 2398..2477 /label=BBa_B0010 stem_loop 2493..2512 /label=T7 TE misc_feature 2519..2519 /label=stop polyA_site 2513..2526 /label=polya misc_feature 2486..2526 /label=BBa_B0012 terminator 2486..2526 /label=BBa_B0012 terminator 2398..2526 /label=BBa_B0015 misc_feature 2537..2553 /label=lac O1 promoter 2554..2559 /label=-35 misc_feature 2560..2576 /label=lac O1 promoter 2577..2582 /label=-10 misc_feature 2535..2588 /label=BBa_R0011 promoter 2535..2588 /label=BBa_R0011 misc_feature 1219..2589 /label=BBa_Q04121 misc_feature 2602..2605 /label=conserved RBS 2598..2609 /label=BBa_B0034 CDS 2616..3377 /label=YFP (LVA) misc_feature 3372..3377 /label=2 misc_feature 3333..3371 /label=SsrA misc_feature 2857..2857 /label=A (Q->L) misc_feature 2616..3377 /label=BBa_E0032 misc_feature 2616..3377 /label=BBa_E0032 stem_loop 3397..3440 /label=stem_loop misc_feature 3386..3465 /label=BBa_B0010 terminator 3386..3465 /label=BBa_B0010 stem_loop 3481..3500 /label=T7 TE misc_feature 3507..3507 /label=stop polyA_site 3501..3514 /label=polya misc_feature 3474..3514 /label=BBa_B0012 terminator 3474..3514 /label=BBa_B0012 misc_feature 2598..3514 /label=BBa_E0432 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg 1261 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 1321 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 1381 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 1441 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 1501 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 1561 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 1621 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 1681 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 1741 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 1801 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 1861 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 1921 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 1981 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt 2041 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 2101 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 2161 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 2221 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 2281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa 2341 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcca 2401 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2461 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2521 tttatatact agagaattgt gagcggataa caattgacat tgtgagcgga taacaagata 2581 ctgagcacat actagagaaa gaggagaaat actagatggt gagcaagggc gaggagctgt 2641 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 2701 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 2761 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc 2821 tgcaatgctt cgcccgctac cccgaccaca tgaagctgca cgacttcttc aagtccgcca 2881 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 2941 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 3001 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 3061 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 3121 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 3181 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga 3241 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 3301 ggatcactct cggcatggac gagctgtaca agaggcctgc tgcaaacgac gaaaactacg 3361 ctttagtagc ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 3421 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 3481 ggctcacctt cgggtgggcc tttctgcgtt tata //