LOCUS BBa_E0602 3130 bp DNA linear 15-Nov-2024 DEFINITION I0500.Q04510.E0432 ACCESSION BBa_E0602 VERSION BBa_E0602.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1240..1947 /label=cI lambda misc_feature 1948..1980 /label=LVA misc_feature 1987..2011 /label=Help:Barcodes CDS 1237..1986 /label=BBa_C0051 stem_loop 2031..2074 /label=stem_loop misc_feature 2020..2099 /label=BBa_B0010 terminator 2020..2099 /label=BBa_B0010 stem_loop 2115..2134 /label=T7 TE misc_feature 2141..2141 /label=stop polyA_site 2135..2148 /label=polya misc_feature 2108..2148 /label=BBa_B0012 terminator 2108..2148 /label=BBa_B0012 promoter 2194..2199 /label=-10 promoter 2171..2176 /label=-35 misc_feature 2181..2197 /label=OR1 misc_feature 2157..2173 /label=OR2 misc_feature 2157..2205 /label=BBa_R0051 promoter 2157..2205 /label=BBa_R0051 misc_feature 2218..2221 /label=conserved RBS 2214..2225 /label=BBa_B0034 CDS 2232..2993 /label=YFP (LVA) misc_feature 2988..2993 /label=2 misc_feature 2949..2987 /label=SsrA misc_feature 2473..2473 /label=A (Q->L) misc_feature 2232..2993 /label=BBa_E0032 misc_feature 2232..2993 /label=BBa_E0032 stem_loop 3013..3056 /label=stem_loop misc_feature 3002..3081 /label=BBa_B0010 terminator 3002..3081 /label=BBa_B0010 stem_loop 3097..3116 /label=T7 TE misc_feature 3123..3123 /label=stop polyA_site 3117..3130 /label=polya misc_feature 3090..3130 /label=BBa_B0012 terminator 3090..3130 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta 1261 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat 1321 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt 1381 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa 1441 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg 1501 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct 1561 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag 1621 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt 1681 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc 1741 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat 1801 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac 1861 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct 1921 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct 1981 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa 2041 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 2101 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtaac 2161 accgtgcgtg ttgactattt tacctctggc ggtgataatg gttgctacta gagaaagagg 2221 agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 2281 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 2341 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 2401 cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc cgctaccccg 2461 accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 2521 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 2581 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 2641 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 2701 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 2761 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 2821 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg 2881 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 2941 tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa taatactaga 3001 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 3061 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 3121 tgcgtttata //