LOCUS BBa_E0603 3178 bp DNA linear 15-Dec-2024 DEFINITION I0500.Q04511.E0432 ACCESSION BBa_E0603 VERSION BBa_E0603.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1240..1947 /label=cI lambda misc_feature 1948..1980 /label=LVA misc_feature 1987..2011 /label=Help:Barcodes CDS 1237..1986 /label=BBa_C0051 stem_loop 2031..2074 /label=stem_loop misc_feature 2020..2099 /label=BBa_B0010 terminator 2020..2099 /label=BBa_B0010 stem_loop 2115..2134 /label=T7 TE misc_feature 2141..2141 /label=stop polyA_site 2135..2148 /label=polya misc_feature 2108..2148 /label=BBa_B0012 terminator 2108..2148 /label=BBa_B0012 misc_feature 2157..2253 /label=BBa_R0065 misc_feature 2162..2181 /label=Lux Box promoter 2203..2208 /label=-10 misc_feature 2213..2229 /label=OR2 cI promoter 2214..2214 /label=lux p(R) start promoter 2227..2232 /label=-35 misc_feature 2237..2253 /label=OR1 cI promoter 2250..2253 /label=-10 promoter 2157..2253 /label=BBa_R0065 misc_feature 2266..2269 /label=conserved RBS 2262..2273 /label=BBa_B0034 CDS 2280..3041 /label=YFP (LVA) misc_feature 3036..3041 /label=2 misc_feature 2997..3035 /label=SsrA misc_feature 2521..2521 /label=A (Q->L) misc_feature 2280..3041 /label=BBa_E0032 misc_feature 2280..3041 /label=BBa_E0032 stem_loop 3061..3104 /label=stem_loop misc_feature 3050..3129 /label=BBa_B0010 terminator 3050..3129 /label=BBa_B0010 stem_loop 3145..3164 /label=T7 TE misc_feature 3171..3171 /label=stop polyA_site 3165..3178 /label=polya misc_feature 3138..3178 /label=BBa_B0012 terminator 3138..3178 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta 1261 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat 1321 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt 1381 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa 1441 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg 1501 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct 1561 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag 1621 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt 1681 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc 1741 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat 1801 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac 1861 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct 1921 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct 1981 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa 2041 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 2101 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtaag 2161 cacctgtagg atcgtacagg tttacgcaag aaaatggttt gttatagtcg aataacaccg 2221 tgcgtgttga ctattttacc tctggcggtg atatactaga gaaagaggag aaatactaga 2281 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg 2341 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg 2401 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc 2461 tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc 2521 tgcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct 2581 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 2641 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca 2701 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg 2761 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg 2821 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact 2881 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc 2941 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagaggc 3001 ctgctgcaaa cgacgaaaac tacgctttag tagcttaata atactagagc caggcatcaa 3061 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 3121 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //