LOCUS BBa_E0604 3046 bp DNA linear 16-Dec-2024 DEFINITION I0500.Q04520.E0432 ACCESSION BBa_E0604 VERSION BBa_E0604.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1905 /label=cI 434 misc_feature 1867..1905 /label=LVA misc_feature 1237..1905 /label=BBa_C0052 misc_feature 1906..1930 /label=Help:Barcodes CDS 1237..1905 /label=BBa_C0052 stem_loop 1950..1993 /label=stem_loop misc_feature 1939..2018 /label=BBa_B0010 terminator 1939..2018 /label=BBa_B0010 stem_loop 2034..2053 /label=T7 TE misc_feature 2060..2060 /label=stop polyA_site 2054..2067 /label=polya misc_feature 2027..2067 /label=BBa_B0012 terminator 2027..2067 /label=BBa_B0012 misc_feature 2076..2118 /label=OR2 misc_feature 2083..2096 /label=OR2 misc_feature 2105..2118 /label=OR1 promoter 2076..2081 /label=-35 promoter 2099..2104 /label=-10 promoter 2077..2082 /label=-35 misc_feature 2110..2112 /label=start misc_feature 2076..2121 /label=BBa_R0052 promoter 2076..2121 /label=BBa_R0052 misc_feature 2134..2137 /label=conserved RBS 2130..2141 /label=BBa_B0034 CDS 2148..2909 /label=YFP (LVA) misc_feature 2904..2909 /label=2 misc_feature 2865..2903 /label=SsrA misc_feature 2389..2389 /label=A (Q->L) misc_feature 2148..2909 /label=BBa_E0032 misc_feature 2148..2909 /label=BBa_E0032 stem_loop 2929..2972 /label=stem_loop misc_feature 2918..2997 /label=BBa_B0010 terminator 2918..2997 /label=BBa_B0010 stem_loop 3013..3032 /label=T7 TE misc_feature 3039..3039 /label=stop polyA_site 3033..3046 /label=polya misc_feature 3006..3046 /label=BBa_B0012 terminator 3006..3046 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga gtatttcttc cagggtaaaa 1261 agcaaaagaa tccagcttgg acttaaccag gctgaacttg ctcaaaaggt ggggactacc 1321 cagcagtcta tagagcagct cgaaaacggt aaaactaagc gaccacgctt tttaccagaa 1381 cttgcgtcag ctcttggcgt aagtgttgac tggctgctca atggcacctc tgattcgaat 1441 gttagatttg ttgggcacgt tgagcccaaa gggaaatatc cattgattag catggttaga 1501 gctggttcgt ggtgtgaagc ttgtgaaccc tacgatatca aggacattga tgaatggtat 1561 gacagtgacg ttaacttatt aggcaatgga ttctggctga aggttgaagg tgattccatg 1621 acctcacctg taggtcaaag catccctgaa ggtcatatgg tgttagtaga tactggacgg 1681 gagccagtga atggaagcct tgttgtagcc aaactgactg acgcgaacga agcaacattc 1741 aagaaactgg tcatagatgg cggtcagaag tacctgaaag gcctgaatcc ttcatggcct 1801 atgactccta tcaacggaaa ctgcaagatt atcggtgttg tcgtggaagc gagggtaaaa 1861 ttcgtagctg caaacgacga aaactacgct ttagtagctt aataactctg atagtgctag 1921 tgtagatctc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 1981 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 2041 cttcgggtgg gcctttctgc gtttatatac tagagttgac aaacaagata cattgtatga 2101 aaatacaaga aagtttgttg atactagaga aagaggagaa atactagatg gtgagcaagg 2161 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 2221 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 2281 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct 2341 tcggctacgg cctgcaatgc ttcgcccgct accccgacca catgaagctg cacgacttct 2401 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 2461 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 2521 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 2581 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 2641 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 2701 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc 2761 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 2821 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagaggcct gctgcaaacg 2881 acgaaaacta cgctttagta gcttaataat actagagcca ggcatcaaat aaaacgaaag 2941 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 3001 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //