LOCUS BBa_E0605 3072 bp DNA linear 16-Dec-2024 DEFINITION I0500.Q04530.E0432 ACCESSION BBa_E0605 VERSION BBa_E0605.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..1884 /label=cII p22 misc_feature 1885..1917 /label=LVA misc_feature 1918..1923 /label=stop misc_feature 1237..1923 /label=BBa_C0053 misc_feature 1924..1948 /label=Help:Barcodes CDS 1237..1923 /label=BBa_C0053 stem_loop 1968..2011 /label=stem_loop misc_feature 1957..2036 /label=BBa_B0010 terminator 1957..2036 /label=BBa_B0010 stem_loop 2052..2071 /label=T7 TE misc_feature 2078..2078 /label=stop polyA_site 2072..2085 /label=polya misc_feature 2045..2085 /label=BBa_B0012 terminator 2045..2085 /label=BBa_B0012 misc_feature 2094..2096 /label=OR3 misc_feature 2104..2121 /label=OR2 misc_feature 2127..2144 /label=OR1 promoter 2111..2116 /label=-35 promoter 2101..2106 /label=-35 promoter 2123..2128 /label=-10 misc_feature 2094..2147 /label=BBa_R0053 promoter 2094..2147 /label=BBa_R0053 misc_feature 2160..2163 /label=conserved RBS 2156..2167 /label=BBa_B0034 CDS 2174..2935 /label=YFP (LVA) misc_feature 2930..2935 /label=2 misc_feature 2891..2929 /label=SsrA misc_feature 2415..2415 /label=A (Q->L) misc_feature 2174..2935 /label=BBa_E0032 misc_feature 2174..2935 /label=BBa_E0032 stem_loop 2955..2998 /label=stem_loop misc_feature 2944..3023 /label=BBa_B0010 terminator 2944..3023 /label=BBa_B0010 stem_loop 3039..3058 /label=T7 TE misc_feature 3065..3065 /label=stop polyA_site 3059..3072 /label=polya misc_feature 3032..3072 /label=BBa_B0012 terminator 3032..3072 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga atacacaatt gatgggtgag 1261 cgtattcgcg ctcgaagaaa aaaactcaag attagacaag ccgctcttgg taagatggtg 1321 ggagtgtcta atgttgcaat atcgcaatgg gagcgctcgg agactgagcc aaatggggag 1381 aacctgttgg cactttcgaa ggctcttcag tgctcccctg actatttgct gaaaggagat 1441 ttaagccaga caaacgttgc ctatcatagt aggcatgagc caagaggatc ataccctctt 1501 atcagttggg taagcgcagg gcaatggatg gaagctgtag aaccttatca caagcgcgcg 1561 atagagaact ggcacgacac cactgtagat tgttcagaag attcattttg gcttgatgtc 1621 caaggtgact ctatgacagc accggcaggg ttaagcattc cagaaggaat gataattctg 1681 gttgatcccg aagtcgaacc aagaaacggc aagctggttg ttgcaaaatt agaaggtgaa 1741 aacgaggcca cattcaaaaa attagttatg gatgcaggcc gaaagttttt aaaaccatta 1801 aacccacaat atccgatgat agaaatcaac ggaaactgca aaatcattgg cgtagttgtt 1861 gacgcaaaac tcgcaaatct tccagctgca aacgacgaaa actacgcttt agtagcttaa 1921 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg 1981 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 2041 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagaataaac 2101 ttgactaaag attcctttag tagataattt aagtgttctt taatttctac tagagaaaga 2161 ggagaaatac tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 2221 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 2281 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 2341 gccctggccc accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc 2401 cgaccacatg aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 2461 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 2521 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 2581 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 2641 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 2701 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 2761 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg 2821 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 2881 gctgtacaag aggcctgctg caaacgacga aaactacgct ttagtagctt aataatacta 2941 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 3001 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 3061 tctgcgttta ta //