LOCUS       BBa_E0613               3139 bp    DNA     linear       12-May-2024
DEFINITION  I0500.Q04511.E0430
ACCESSION   BBa_E0613
VERSION     BBa_E0613.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1240..1947
                     /label=cI lambda
     misc_feature    1948..1980
                     /label=LVA
     misc_feature    1987..2011
                     /label=Help:Barcodes
     CDS             1237..1986
                     /label=BBa_C0051
     stem_loop       2031..2074
                     /label=stem_loop
     misc_feature    2020..2099
                     /label=BBa_B0010
     terminator      2020..2099
                     /label=BBa_B0010
     stem_loop       2115..2134
                     /label=T7 TE
     misc_feature    2141..2141
                     /label=stop
     polyA_site      2135..2148
                     /label=polya
     misc_feature    2108..2148
                     /label=BBa_B0012
     terminator      2108..2148
                     /label=BBa_B0012
     misc_feature    2157..2253
                     /label=BBa_R0065
     misc_feature    2162..2181
                     /label=Lux Box
     promoter        2203..2208
                     /label=-10
     misc_feature    2213..2229
                     /label=OR2 cI
     promoter        2214..2214
                     /label=lux p(R) start
     promoter        2227..2232
                     /label=-35
     misc_feature    2237..2253
                     /label=OR1 cI
     promoter        2250..2253
                     /label=-10
     promoter        2157..2253
                     /label=BBa_R0065
     misc_feature    2266..2269
                     /label=conserved
     RBS             2262..2273
                     /label=BBa_B0034
     CDS             2280..3002
                     /label=BBa_E0030
     stem_loop       3022..3065
                     /label=stem_loop
     misc_feature    3011..3090
                     /label=BBa_B0010
     terminator      3011..3090
                     /label=BBa_B0010
     stem_loop       3106..3125
                     /label=T7 TE
     misc_feature    3132..3132
                     /label=stop
     polyA_site      3126..3139
                     /label=polya
     misc_feature    3099..3139
                     /label=BBa_B0012
     terminator      3099..3139
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta
     1261 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat
     1321 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt
     1381 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa
     1441 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg
     1501 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct
     1561 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag
     1621 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt
     1681 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc
     1741 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat
     1801 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac
     1861 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct
     1921 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct
     1981 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa
     2041 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     2101 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtaag
     2161 cacctgtagg atcgtacagg tttacgcaag aaaatggttt gttatagtcg aataacaccg
     2221 tgcgtgttga ctattttacc tctggcggtg atatactaga gaaagaggag aaatactaga
     2281 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     2341 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     2401 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     2461 tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc
     2521 tgcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     2581 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     2641 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     2701 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     2761 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     2821 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     2881 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     2941 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat
     3001 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     3061 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     3121 gggcctttct gcgtttata
//