LOCUS       BBa_E0614               3007 bp    DNA     linear       25-Nov-2024
DEFINITION  I0500.Q04520.E0430
ACCESSION   BBa_E0614
VERSION     BBa_E0614.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1905
                     /label=cI 434
     misc_feature    1867..1905
                     /label=LVA
     misc_feature    1237..1905
                     /label=BBa_C0052
     misc_feature    1906..1930
                     /label=Help:Barcodes
     CDS             1237..1905
                     /label=BBa_C0052
     stem_loop       1950..1993
                     /label=stem_loop
     misc_feature    1939..2018
                     /label=BBa_B0010
     terminator      1939..2018
                     /label=BBa_B0010
     stem_loop       2034..2053
                     /label=T7 TE
     misc_feature    2060..2060
                     /label=stop
     polyA_site      2054..2067
                     /label=polya
     misc_feature    2027..2067
                     /label=BBa_B0012
     terminator      2027..2067
                     /label=BBa_B0012
     misc_feature    2076..2118
                     /label=OR2
     misc_feature    2083..2096
                     /label=OR2
     misc_feature    2105..2118
                     /label=OR1
     promoter        2076..2081
                     /label=-35
     promoter        2099..2104
                     /label=-10
     promoter        2077..2082
                     /label=-35
     misc_feature    2110..2112
                     /label=start
     misc_feature    2076..2121
                     /label=BBa_R0052
     promoter        2076..2121
                     /label=BBa_R0052
     misc_feature    2134..2137
                     /label=conserved
     RBS             2130..2141
                     /label=BBa_B0034
     CDS             2148..2870
                     /label=BBa_E0030
     stem_loop       2890..2933
                     /label=stem_loop
     misc_feature    2879..2958
                     /label=BBa_B0010
     terminator      2879..2958
                     /label=BBa_B0010
     stem_loop       2974..2993
                     /label=T7 TE
     misc_feature    3000..3000
                     /label=stop
     polyA_site      2994..3007
                     /label=polya
     misc_feature    2967..3007
                     /label=BBa_B0012
     terminator      2967..3007
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga gtatttcttc cagggtaaaa
     1261 agcaaaagaa tccagcttgg acttaaccag gctgaacttg ctcaaaaggt ggggactacc
     1321 cagcagtcta tagagcagct cgaaaacggt aaaactaagc gaccacgctt tttaccagaa
     1381 cttgcgtcag ctcttggcgt aagtgttgac tggctgctca atggcacctc tgattcgaat
     1441 gttagatttg ttgggcacgt tgagcccaaa gggaaatatc cattgattag catggttaga
     1501 gctggttcgt ggtgtgaagc ttgtgaaccc tacgatatca aggacattga tgaatggtat
     1561 gacagtgacg ttaacttatt aggcaatgga ttctggctga aggttgaagg tgattccatg
     1621 acctcacctg taggtcaaag catccctgaa ggtcatatgg tgttagtaga tactggacgg
     1681 gagccagtga atggaagcct tgttgtagcc aaactgactg acgcgaacga agcaacattc
     1741 aagaaactgg tcatagatgg cggtcagaag tacctgaaag gcctgaatcc ttcatggcct
     1801 atgactccta tcaacggaaa ctgcaagatt atcggtgttg tcgtggaagc gagggtaaaa
     1861 ttcgtagctg caaacgacga aaactacgct ttagtagctt aataactctg atagtgctag
     1921 tgtagatctc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1981 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     2041 cttcgggtgg gcctttctgc gtttatatac tagagttgac aaacaagata cattgtatga
     2101 aaatacaaga aagtttgttg atactagaga aagaggagaa atactagatg gtgagcaagg
     2161 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     2221 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2281 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct
     2341 tcggctacgg cctgcaatgc ttcgcccgct accccgacca catgaagctg cacgacttct
     2401 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2461 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2521 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2581 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     2641 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     2701 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc
     2761 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     2821 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaataa tactagagcc
     2881 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2941 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     3001 gtttata
//