LOCUS       BBa_E0615               3033 bp    DNA     linear       07-May-2024
DEFINITION  I0500.Q04530.E0430
ACCESSION   BBa_E0615
VERSION     BBa_E0615.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1884
                     /label=cII p22
     misc_feature    1885..1917
                     /label=LVA
     misc_feature    1918..1923
                     /label=stop
     misc_feature    1237..1923
                     /label=BBa_C0053
     misc_feature    1924..1948
                     /label=Help:Barcodes
     CDS             1237..1923
                     /label=BBa_C0053
     stem_loop       1968..2011
                     /label=stem_loop
     misc_feature    1957..2036
                     /label=BBa_B0010
     terminator      1957..2036
                     /label=BBa_B0010
     stem_loop       2052..2071
                     /label=T7 TE
     misc_feature    2078..2078
                     /label=stop
     polyA_site      2072..2085
                     /label=polya
     misc_feature    2045..2085
                     /label=BBa_B0012
     terminator      2045..2085
                     /label=BBa_B0012
     misc_feature    2094..2096
                     /label=OR3
     misc_feature    2104..2121
                     /label=OR2
     misc_feature    2127..2144
                     /label=OR1
     promoter        2111..2116
                     /label=-35
     promoter        2101..2106
                     /label=-35
     promoter        2123..2128
                     /label=-10
     misc_feature    2094..2147
                     /label=BBa_R0053
     promoter        2094..2147
                     /label=BBa_R0053
     misc_feature    2160..2163
                     /label=conserved
     RBS             2156..2167
                     /label=BBa_B0034
     CDS             2174..2896
                     /label=BBa_E0030
     stem_loop       2916..2959
                     /label=stem_loop
     misc_feature    2905..2984
                     /label=BBa_B0010
     terminator      2905..2984
                     /label=BBa_B0010
     stem_loop       3000..3019
                     /label=T7 TE
     misc_feature    3026..3026
                     /label=stop
     polyA_site      3020..3033
                     /label=polya
     misc_feature    2993..3033
                     /label=BBa_B0012
     terminator      2993..3033
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga atacacaatt gatgggtgag
     1261 cgtattcgcg ctcgaagaaa aaaactcaag attagacaag ccgctcttgg taagatggtg
     1321 ggagtgtcta atgttgcaat atcgcaatgg gagcgctcgg agactgagcc aaatggggag
     1381 aacctgttgg cactttcgaa ggctcttcag tgctcccctg actatttgct gaaaggagat
     1441 ttaagccaga caaacgttgc ctatcatagt aggcatgagc caagaggatc ataccctctt
     1501 atcagttggg taagcgcagg gcaatggatg gaagctgtag aaccttatca caagcgcgcg
     1561 atagagaact ggcacgacac cactgtagat tgttcagaag attcattttg gcttgatgtc
     1621 caaggtgact ctatgacagc accggcaggg ttaagcattc cagaaggaat gataattctg
     1681 gttgatcccg aagtcgaacc aagaaacggc aagctggttg ttgcaaaatt agaaggtgaa
     1741 aacgaggcca cattcaaaaa attagttatg gatgcaggcc gaaagttttt aaaaccatta
     1801 aacccacaat atccgatgat agaaatcaac ggaaactgca aaatcattgg cgtagttgtt
     1861 gacgcaaaac tcgcaaatct tccagctgca aacgacgaaa actacgcttt agtagcttaa
     1921 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg
     1981 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     2041 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagaataaac
     2101 ttgactaaag attcctttag tagataattt aagtgttctt taatttctac tagagaaaga
     2161 ggagaaatac tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2221 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     2281 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     2341 gccctggccc accctcgtga ccaccttcgg ctacggcctg caatgcttcg cccgctaccc
     2401 cgaccacatg aagctgcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     2461 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     2521 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     2581 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     2641 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     2701 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2761 gcccgacaac cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg
     2821 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     2881 gctgtacaag taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     2941 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     3001 gctcaccttc gggtgggcct ttctgcgttt ata
//