LOCUS BBa_E0669 2815 bp DNA linear 10-May-2024 DEFINITION CRY-FP output plasmid v2 ACCESSION BBa_E0669 VERSION BBa_E0669.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 misc_feature 62..65 /label=conserved RBS 58..69 /label=BBa_B0034 CDS 76..798 /label=BBa_E0030 stem_loop 818..861 /label=stem_loop misc_feature 807..886 /label=BBa_B0010 terminator 807..886 /label=BBa_B0010 stem_loop 902..921 /label=T7 TE misc_feature 928..928 /label=stop polyA_site 922..935 /label=polya misc_feature 895..935 /label=BBa_B0012 terminator 895..935 /label=BBa_B0012 misc_feature 58..935 /label=BBa_E0430 misc_feature 946..962 /label=lac O1 promoter 963..968 /label=-35 misc_feature 969..985 /label=lac O1 promoter 986..991 /label=-10 misc_feature 944..997 /label=BBa_R0011 promoter 944..997 /label=BBa_R0011 misc_feature 1011..1014 /label=conserved RBS 1007..1018 /label=BBa_B0034 CDS 1025..1747 /label=BBa_E0020 stem_loop 1767..1810 /label=stem_loop misc_feature 1756..1835 /label=BBa_B0010 terminator 1756..1835 /label=BBa_B0010 stem_loop 1851..1870 /label=T7 TE misc_feature 1877..1877 /label=stop polyA_site 1871..1884 /label=polya misc_feature 1844..1884 /label=BBa_B0012 terminator 1844..1884 /label=BBa_B0012 misc_feature 1007..1884 /label=BBa_E0420 misc_feature 1893..1911 /label=TetR 1 misc_feature 1893..1946 /label=BBa_R0040 promoter 1912..1917 /label=-35 misc_feature 1918..1936 /label=TetR 2 promoter 1935..1940 /label=-10 promoter 1893..1946 /label=BBa_R0040 misc_feature 1959..1962 /label=conserved RBS 1955..1966 /label=BBa_B0034 CDS 1973..2647 /label=mrfp1 misc_feature 2654..2678 /label=Help:Barcodes CDS 1973..2653 /label=BBa_E1010 stem_loop 2698..2741 /label=stem_loop misc_feature 2687..2766 /label=BBa_B0010 terminator 2687..2766 /label=BBa_B0010 stem_loop 2782..2801 /label=T7 TE misc_feature 2808..2808 /label=stop polyA_site 2802..2815 /label=polya misc_feature 2775..2815 /label=BBa_B0012 terminator 2775..2815 /label=BBa_B0012 misc_feature 1955..2815 /label=BBa_E041010 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa 61 gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc 121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag 181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc 241 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt cgcccgctac 301 cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag 361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc 421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc 481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc 541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc 601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 661 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag 721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 781 gagctgtaca agtaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 841 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 901 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagaattgtg agcggataac 961 aattgacatt gtgagcggat aacaagatac tgagcacata ctagagaaag aggagaaata 1021 ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct 1081 ggacggcgac gtgaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac 1141 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc 1201 caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat 1261 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat 1321 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac 1381 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 1441 gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa 1501 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct 1561 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 1621 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat 1681 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa 1741 gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 1801 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 1861 cgggtgggcc tttctgcgtt tatatactag agtccctatc agtgatagag attgacatcc 1921 ctatcagtga tagagatact gagcactact agagaaagag gagaaatact agatggcttc 1981 ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa 2041 cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac 2101 cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc 2161 gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct 2221 gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg 2281 tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa 2341 actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg 2401 ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat 2461 gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc 2521 taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc 2581 ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac 2641 cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata 2701 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 2761 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //