##gff-version 3
##sequence-region BBa_E0669 1 2815
BBa_E0669	.	engineered_region	1955	2815	.	+	0	ID=BBa_E041010;Name=BBa_E041010
BBa_E0669	.	ribosome_entry_site	1955	1966	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E041010
BBa_E0669	.	conserved_region	1959	1962	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_E0669	.	CDS	1973	2653	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_E041010
BBa_E0669	.	CDS	1973	2647	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_E0669	.	engineered_tag	2654	2678	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_E0669	.	terminator	2775	2815	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E041010
BBa_E0669	.	engineered_region	2775	2815	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_E0669	.	stem_loop	2782	2801	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_E0669	.	stop_codon	2808	2808	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_E0669	.	polyA_site	2802	2815	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_E0669	.	terminator	2687	2766	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E041010
BBa_E0669	.	engineered_region	2687	2766	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_E0669	.	stem_loop	2698	2741	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_E0669	.	engineered_region	1007	1884	.	+	0	ID=BBa_E0420;Name=BBa_E0420
BBa_E0669	.	ribosome_entry_site	1007	1018	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0420
BBa_E0669	.	conserved_region	1011	1014	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_E0669	.	terminator	1756	1835	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0420
BBa_E0669	.	engineered_region	1756	1835	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_E0669	.	stem_loop	1767	1810	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_E0669	.	terminator	1844	1884	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0420
BBa_E0669	.	engineered_region	1844	1884	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_E0669	.	stem_loop	1851	1870	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_E0669	.	stop_codon	1877	1877	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_E0669	.	polyA_site	1871	1884	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_E0669	.	CDS	1025	1747	.	+	0	ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_E0420
BBa_E0669	.	promoter	944	997	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_E0669	.	operator	969	985	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_E0669	.	operator	946	962	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_E0669	.	promoter	986	991	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_E0669	.	engineered_region	944	997	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_E0669	.	promoter	963	968	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_E0669	.	promoter	1893	1946	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_E0669	.	promoter	1935	1940	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_E0669	.	promoter	1912	1917	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_E0669	.	engineered_region	1893	1946	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_E0669	.	non_covalent_binding_site	1918	1936	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_E0669	.	non_covalent_binding_site	1893	1911	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_E0669	.	engineered_region	58	935	.	+	0	ID=BBa_E0430;Name=BBa_E0430
BBa_E0669	.	terminator	807	886	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0430
BBa_E0669	.	engineered_region	807	886	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_E0669	.	stem_loop	818	861	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_E0669	.	terminator	895	935	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0430
BBa_E0669	.	engineered_region	895	935	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_E0669	.	stem_loop	902	921	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_E0669	.	stop_codon	928	928	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_E0669	.	polyA_site	922	935	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_E0669	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0430
BBa_E0669	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_E0669	.	CDS	76	798	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_E0430
BBa_E0669	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_E0669	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_E0669	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_E0669	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_E0669	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_E0669	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
>BBa_E0669
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgg
tgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagtt
cagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctg
cccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagc
tgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaacta
caagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggag
gacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaaga
acggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaa
cacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagacccc
aacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtaca
agtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattg
tgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatg
gtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagt
tcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagct
gcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaag
cagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaact
acaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaagga
ggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaag
aacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcaga
acacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccc
caacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtac
aagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccc
tatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatg
gcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcg
aaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgct
gccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccg
gactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccg
ttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacgg
tccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggt
gaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaac
cggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttga
acagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata