LOCUS BBa_E5503 803 bp DNA linear 22-Dec-2024 DEFINITION R0040.B0030.E0040 ACCESSION BBa_E5503 VERSION BBa_E5503.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..77 /label=RBS-1\Strong RBS 70..74 /label=RBS misc_feature 63..77 /label=BBa_B0030 RBS 63..77 /label=BBa_B0030 CDS 84..803 /label=GFP protein CDS 84..803 /label=BBa_E0040 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agattaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc 121 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg 181 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac 241 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga 301 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg 361 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca 421 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag 481 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca 541 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag 601 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg 661 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg 721 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca 781 tggatgaact atacaaataa taa //