LOCUS       BBa_E70101               935 bp    DNA     linear       14-May-2024
DEFINITION  GFP expression system that uses dedicated translation
ACCESSION   BBa_E70101
VERSION     BBa_E70101.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..72
                     /label=BBa_B0037
     CDS             79..798
                     /label=GFP protein
     CDS             79..798
                     /label=BBa_E0040
     stem_loop       818..861
                     /label=stem_loop
     misc_feature    807..886
                     /label=BBa_B0010
     terminator      807..886
                     /label=BBa_B0010
     stem_loop       902..921
                     /label=T7 TE
     misc_feature    928..928
                     /label=stop
     polyA_site      922..935
                     /label=polya
     misc_feature    895..935
                     /label=BBa_B0012
     terminator      895..935
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 aggtgtgtct agtactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
      121 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      181 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      241 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      301 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      361 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      421 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      481 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      541 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      601 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      661 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      721 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      781 gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      841 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      901 tggctcacct tcgggtgggc ctttctgcgt ttata
//