LOCUS       BBa_E70102               941 bp    DNA     linear       22-Dec-2024
DEFINITION  Reporter for dedicated translation
ACCESSION   BBa_E70102
VERSION     BBa_E70102.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..78
                     /label=BBa_B0070
     CDS             85..804
                     /label=GFP protein
     CDS             85..804
                     /label=BBa_E0040
     stem_loop       824..867
                     /label=stem_loop
     misc_feature    813..892
                     /label=BBa_B0010
     terminator      813..892
                     /label=BBa_B0010
     stem_loop       908..927
                     /label=T7 TE
     misc_feature    934..934
                     /label=stop
     polyA_site      928..941
                     /label=polya
     misc_feature    901..941
                     /label=BBa_B0012
     terminator      901..941
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtctcacgt gtgtcaagta ctagatgcgt aaaggagaag aacttttcac tggagttgtc
      121 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
      181 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
      241 ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg
      301 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat
      361 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc
      421 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
      481 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
      541 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa
      601 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
      661 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac
      721 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc
      781 atggatgaac tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca
      841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      901 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//