LOCUS       BBa_E70202               934 bp    DNA     linear       02-May-2024
DEFINITION  Reporter for Dedicated Translation
ACCESSION   BBa_E70202
VERSION     BBa_E70202.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..71
                     /label=BBa_B0073
     CDS             78..797
                     /label=GFP protein
     CDS             78..797
                     /label=BBa_E0040
     stem_loop       817..860
                     /label=stem_loop
     misc_feature    806..885
                     /label=BBa_B0010
     terminator      806..885
                     /label=BBa_B0010
     stem_loop       901..920
                     /label=T7 TE
     misc_feature    927..927
                     /label=stop
     polyA_site      921..934
                     /label=polya
     misc_feature    894..934
                     /label=BBa_B0012
     terminator      894..934
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacca ctactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc
      121 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      181 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      241 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc
      301 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      361 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
      421 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
      481 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
      541 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
      601 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
      661 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
      721 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
      781 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
      841 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
      901 ggctcacctt cgggtgggcc tttctgcgtt tata
//