BBa_G0007 1 BBa_G0007 Short sequence to promoter addition of 3' A overhang during PCR 2007-07-22T11:00:00Z 2015-08-31T04:07:27Z <biblio> #Brownstein-Biotechniques-1996 pmid=8780871 </biblio> This is a short sequence to promoter addition of 3' A overhang during PCR. It should be added to the 5' end of any primers in which the PCR product is going to be TA cloned. It is also a helpful spacer sequence when generating linear DNA fragments to ensure that restriction enzymes will still be able to cut. false false _41_ 0 126 162 Not in stock false None. false Reshma Shetty BBa_G0007_sequence 1 gtttcttc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z