LOCUS       BBa_I0040                596 bp    DNA     linear       22-Dec-2024
DEFINITION  tetR-regulatable expression of lacZalpha
ACCESSION   BBa_I0040
VERSION     BBa_I0040.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..95
                     /label=lacZ gene fragment
     CDS             81..434
                     /label=lacZ alpha
     promoter        106..115
                     /label=T3 promoter
     promoter        253..271
                     /label=T7 promoter
     misc_feature    158..158
                     /label=C
     misc_feature    161..161
                     /label=T
     misc_feature    167..167
                     /label=A
     misc_feature    188..188
                     /label=C
     misc_feature    191..191
                     /label=G
     misc_feature    279..428
                     /label=lacZ gene fragment
     misc_feature    428..428
                     /label=inserted T
     misc_feature    435..459
                     /label=barcode
     misc_feature    81..459
                     /label=BBa_E0035
     stem_loop       479..522
                     /label=stem_loop
     misc_feature    468..547
                     /label=BBa_B0010
     terminator      468..547
                     /label=BBa_B0010
     stem_loop       563..582
                     /label=T7 TE
     misc_feature    589..589
                     /label=stop
     polyA_site      583..596
                     /label=polya
     misc_feature    556..596
                     /label=BBa_B0012
     terminator      556..596
                     /label=BBa_B0012
     terminator      468..596
                     /label=BBa_B0015
     misc_feature    63..596
                     /label=BBa_E0435
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaccatga ttacgccaag cgcgcaatta accctcacta
      121 aagggaacaa aagctggagc tccaccgcgg tggcggcagc actagagcta gtggatcccc
      181 cgggctgtag aaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg
      241 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
      301 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      361 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      421 gcctgaatta ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat
      481 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      541 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//