##gff-version 3
##sequence-region BBa_I0040 1 596
BBa_I0040	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I0040	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I0040	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I0040	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I0040	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I0040	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I0040	.	engineered_region	63	596	.	+	0	ID=BBa_E0435;Name=BBa_E0435
BBa_I0040	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0435
BBa_I0040	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I0040	.	terminator	468	596	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_E0435
BBa_I0040	.	terminator	556	596	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I0040	.	stem_loop	563	582	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I0040	.	stop_codon	589	589	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I0040	.	polyA_site	583	596	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I0040	.	engineered_region	556	596	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I0040	.	terminator	468	547	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I0040	.	stem_loop	479	522	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I0040	.	engineered_region	468	547	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I0040	.	engineered_region	81	459	.	+	0	ID=BBa_E0035;Name=BBa_E0035;Parent=BBa_E0435
BBa_I0040	.	sequence_alteration	191	191	.	+	0	ID=annotation1959043;Name=G;Parent=BBa_E0035
BBa_I0040	.	sequence_alteration	428	428	.	+	0	ID=annotation1959045;Name=inserted T;Parent=BBa_E0035
BBa_I0040	.	CDS	81	434	.	+	0	ID=annotation1959036;Name=lacZ alpha;Parent=BBa_E0035
BBa_I0040	.	promoter	253	271	.	+	0	ID=annotation1959038;Name=T7 promoter;Parent=BBa_E0035
BBa_I0040	.	sequence_alteration	167	167	.	+	0	ID=annotation1959041;Name=A;Parent=BBa_E0035
BBa_I0040	.	sequence_feature	279	428	.	+	0	ID=annotation1959044;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_I0040	.	sequence_alteration	161	161	.	+	0	ID=annotation1959040;Name=T;Parent=BBa_E0035
BBa_I0040	.	sequence_alteration	158	158	.	+	0	ID=annotation1959039;Name=C;Parent=BBa_E0035
BBa_I0040	.	sequence_alteration	188	188	.	+	0	ID=annotation1959042;Name=C;Parent=BBa_E0035
BBa_I0040	.	sequence_feature	81	95	.	+	0	ID=annotation1959035;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_I0040	.	promoter	106	115	.	+	0	ID=annotation1959037;Name=T3 promoter;Parent=BBa_E0035
BBa_I0040	.	engineered_tag	435	459	.	+	0	ID=annotation1959046;Name=barcode;Parent=BBa_E0035
>BBa_I0040
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggca
gcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcc
cggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaa
ccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcacc
gatcgcccttcccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatcgctactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttata