BBa_I0040
type: ComponentDefinition
persistent identity: BBa I0040
display id: BBa_I0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetR-regulatable expression of lacZalpha
created: 2008-05-05T11:00:00Z
modified: 2015-08-31T04:07:28Z
mutable provenance: Composite part.
owned by: james
owned by: myers
mutable description: tetR-regulatable expression of lacZalpha
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_E0435
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_E0435
sequence: https://synbiohub.org/public/igem/BBa_I0040_sequence/1

BBa_E0435
type: Component
persistent identity: component2253549
display id: component2253549
version: 1
top level: BBa_I0040
definition: BBa_E0435
access: public

BBa_R0040
type: Component
persistent identity: component2253522
display id: component2253522
version: 1
top level: BBa_I0040
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2253522
display id: annotation2253522
version: 1
top level: BBa_I0040
location: https://synbiohub.org/public/igem/BBa_I0040/annotation2253522/range2253522/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I0040/annotation2253522/range2253522/1
type: Range
persistent identity: range2253522
display id: range2253522
version: 1
top level: BBa_I0040
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_E0435
type: SequenceAnnotation
persistent identity: annotation2253549
display id: annotation2253549
version: 1
top level: BBa_I0040
location: https://synbiohub.org/public/igem/BBa_I0040/annotation2253549/range2253549/1
component: BBa_E0435

https://synbiohub.org/public/igem/BBa_I0040/annotation2253549/range2253549/1
type: Range
persistent identity: range2253549
display id: range2253549
version: 1
top level: BBa_I0040
direction: direction/reverse
start: 63
end: 596
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_E0435
type: ComponentDefinition
persistent identity: BBa E0435
display id: BBa_E0435
version: 1
was generated by: iGEM to SBOL conversion
description: PoPS->lacZalpha reporter device
created: 2007-12-21T12:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: It is derived from BBa_E0433.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This device takes a PoPS signal in and produces the protein lacZalpha. When put in a strain that can provide alpha-complementation, you should see measurable beta-galactosidase activity.
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An additional T was inserted to ensure that the double stop codon is in frame with the rest of the protein.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0015
component: BBa_E0035
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_E0035
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_E0435_sequence/1

BBa_B0015
type: Component
persistent identity: component2249043
display id: component2249043
version: 1
top level: BBa_E0435
definition: BBa_B0015
access: public

BBa_E0035
type: Component
persistent identity: component2249036
display id: component2249036
version: 1
top level: BBa_E0435
definition: BBa_E0035
access: public

BBa_B0034
type: Component
persistent identity: component2249023
display id: component2249023
version: 1
top level: BBa_E0435
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2249023
display id: annotation2249023
version: 1
top level: BBa_E0435
location: https://synbiohub.org/public/igem/BBa_E0435/annotation2249023/range2249023/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_E0435/annotation2249023/range2249023/1
type: Range
persistent identity: range2249023
display id: range2249023
version: 1
top level: BBa_E0435
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E0035
type: SequenceAnnotation
persistent identity: annotation2249036
display id: annotation2249036
version: 1
top level: BBa_E0435
location: https://synbiohub.org/public/igem/BBa_E0435/annotation2249036/range2249036/1
component: BBa_E0035

https://synbiohub.org/public/igem/BBa_E0435/annotation2249036/range2249036/1
type: Range
persistent identity: range2249036
display id: range2249036
version: 1
top level: BBa_E0435
direction: direction/reverse
start: 19
end: 397
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2249043
display id: annotation2249043
version: 1
top level: BBa_E0435
location: https://synbiohub.org/public/igem/BBa_E0435/annotation2249043/range2249043/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_E0435/annotation2249043/range2249043/1
type: Range
persistent identity: range2249043
display id: range2249043
version: 1
top level: BBa_E0435
direction: direction/reverse
start: 406
end: 534
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_E0035
type: ComponentDefinition
persistent identity: BBa E0035
display id: BBa_E0035
version: 1
was generated by: iGEM to SBOL conversion
description: LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)
created: 2007-12-21T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: This part is derived from BBa_E0033.
owned by: james
owned by: myers
mutable description: This part encodes the alpha fragment of the lacZ gene. To greatly reduce the number of bases, this is only a portion of the LacZ gene. It can be used as a detection system with N-terminal deletion mutants of lacZ. Combination of lacZ-alpha with such a deletion mutant protein restores enzyme activity that can be assayed colorimetrically or more sensitively with chemiluminescent substrates.
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It has the additional T needed to ensure that the double stop codon is in frame.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: lacZ gene fragment
sequence annotation: lacZ alpha
sequence annotation: T3 promoter
sequence annotation: C
sequence annotation: T
sequence annotation: A
sequence annotation: G
sequence annotation: T7 promoter
sequence annotation: inserted T
sequence annotation: barcode
sequence: https://synbiohub.org/public/igem/BBa_E0035_sequence/1

lacZ gene fragment
type: SequenceAnnotation
persistent identity: annotation1959035
display id: annotation1959035
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959035/range1959035/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_E0035/annotation1959035/range1959035/1
type: Range
persistent identity: range1959035
display id: range1959035
version: 1
top level: BBa_E0035
direction: direction/forward
start: 1
end: 15
orientation: inline

lacZ alpha
type: SequenceAnnotation
persistent identity: annotation1959036
display id: annotation1959036
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959036/range1959036/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0035/annotation1959036/range1959036/1
type: Range
persistent identity: range1959036
display id: range1959036
version: 1
top level: BBa_E0035
direction: direction/forward
start: 1
end: 354
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1959037
display id: annotation1959037
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959037/range1959037/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_E0035/annotation1959037/range1959037/1
type: Range
persistent identity: range1959037
display id: range1959037
version: 1
top level: BBa_E0035
direction: direction/forward
start: 26
end: 35
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1959039
display id: annotation1959039
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959039/range1959039/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0035/annotation1959039/range1959039/1
type: Range
persistent identity: range1959039
display id: range1959039
version: 1
top level: BBa_E0035
direction: direction/forward
start: 78
end: 78
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1959040
display id: annotation1959040
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959040/range1959040/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0035/annotation1959040/range1959040/1
type: Range
persistent identity: range1959040
display id: range1959040
version: 1
top level: BBa_E0035
direction: direction/forward
start: 81
end: 81
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1959041
display id: annotation1959041
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959041/range1959041/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0035/annotation1959041/range1959041/1
type: Range
persistent identity: range1959041
display id: range1959041
version: 1
top level: BBa_E0035
direction: direction/forward
start: 87
end: 87
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1959042
display id: annotation1959042
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959042/range1959042/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_E0035/annotation1959042/range1959042/1
type: Range
persistent identity: range1959042
display id: range1959042
version: 1
top level: BBa_E0035
direction: direction/forward
start: 108
end: 108
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation1959043
display id: annotation1959043
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959043/range1959043/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_E0035/annotation1959043/range1959043/1
type: Range
persistent identity: range1959043
display id: range1959043
version: 1
top level: BBa_E0035
direction: direction/forward
start: 111
end: 111
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1959038
display id: annotation1959038
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959038/range1959038/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_E0035/annotation1959038/range1959038/1
type: Range
persistent identity: range1959038
display id: range1959038
version: 1
top level: BBa_E0035
direction: direction/forward
start: 173
end: 191
orientation: inline

lacZ gene fragment
type: SequenceAnnotation
persistent identity: annotation1959044
display id: annotation1959044
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959044/range1959044/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_E0035/annotation1959044/range1959044/1
type: Range
persistent identity: range1959044
display id: range1959044
version: 1
top level: BBa_E0035
direction: direction/forward
start: 199
end: 348
orientation: inline

inserted T
type: SequenceAnnotation
persistent identity: annotation1959045
display id: annotation1959045
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959045/range1959045/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_E0035/annotation1959045/range1959045/1
type: Range
persistent identity: range1959045
display id: range1959045
version: 1
top level: BBa_E0035
direction: direction/forward
start: 348
end: 348
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1959046
display id: annotation1959046
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959046/range1959046/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_E0035/annotation1959046/range1959046/1
type: Range
persistent identity: range1959046
display id: range1959046
version: 1
top level: BBa_E0035
direction: direction/forward
start: 355
end: 379
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_I0040_sequence/1
type: Sequence
persistent identity: BBa I0040 sequence
display id: BBa_I0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0435_sequence/1
type: Sequence
persistent identity: BBa E0435 sequence
display id: BBa_E0435_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0035_sequence/1
type: Sequence
persistent identity: BBa E0035 sequence
display id: BBa_E0035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z