LOCUS       BBa_I0401               1747 bp    DNA     linear       22-Dec-2024
DEFINITION  R0040.B0034.C0062.E0420
ACCESSION   BBa_I0401
VERSION     BBa_I0401.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     misc_feature    874..877
                     /label=conserved
     RBS             870..881
                     /label=BBa_B0034
     CDS             888..1610
                     /label=BBa_E0020
     stem_loop       1630..1673
                     /label=stem_loop
     misc_feature    1619..1698
                     /label=BBa_B0010
     terminator      1619..1698
                     /label=BBa_B0010
     stem_loop       1714..1733
                     /label=T7 TE
     misc_feature    1740..1740
                     /label=stop
     polyA_site      1734..1747
                     /label=polya
     misc_feature    1707..1747
                     /label=BBa_B0012
     terminator      1707..1747
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagaga aagaggagaa atactagatg gtgagcaagg
      901 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg
      961 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1021 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     1081 tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     1141 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1201 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1261 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1321 actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
     1381 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     1441 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     1501 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1561 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaataa tactagagcc
     1621 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1681 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1741 gtttata
//