LOCUS       BBa_I0402               1609 bp    DNA     linear       22-Nov-2024
DEFINITION  R0040.B0034.C0061.E0420
ACCESSION   BBa_I0402
VERSION     BBa_I0402.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    660..691
                     /label=LVA
     CDS             81..659
                     /label=luxI
     misc_feature    81..698
                     /label=BBa_C0061
     misc_feature    699..723
                     /label=Help:Barcodes
     CDS             81..698
                     /label=BBa_C0061
     misc_feature    736..739
                     /label=conserved
     RBS             732..743
                     /label=BBa_B0034
     CDS             750..1472
                     /label=BBa_E0020
     stem_loop       1492..1535
                     /label=stem_loop
     misc_feature    1481..1560
                     /label=BBa_B0010
     terminator      1481..1560
                     /label=BBa_B0010
     stem_loop       1576..1595
                     /label=T7 TE
     misc_feature    1602..1602
                     /label=stop
     polyA_site      1596..1609
                     /label=polya
     misc_feature    1569..1609
                     /label=BBa_B0012
     terminator      1569..1609
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgactataa tgataaaaaa atcggatttt ttggcaattc
      121 catcggagga gtataaaggt attctaagtc ttcgttatca agtgtttaag caaagacttg
      181 agtgggactt agttgtagaa aataaccttg aatcagatga gtatgataac tcaaatgcag
      241 aatatattta tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt ttattaccta
      301 caacaggtga ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc
      361 ccaaagatcc taatatagtc gaattaagtc gttttgctgt aggtaaaaat agctcaaaga
      421 taaataactc tgctagtgaa attacaatga aactatttga agctatatat aaacacgctg
      481 ttagtcaagg tattacagaa tatgtaacag taacatcaac agcaatagag cgatttttaa
      541 agcgtattaa agttccttgt catcgtattg gagacaaaga aattcatgta ttaggtgata
      601 ctaaatcggt tgtattgtct atgcctatta atgaacagtt taaaaaagca gtcttaaatg
      661 ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat
      721 ctctactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg
      781 gggtggtgcc catcctggtc gagctggacg gcgacgtgaa cggccacaag ttcagcgtgt
      841 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      901 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt
      961 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1021 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1081 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1141 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc agccacaacg
     1201 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
     1261 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1321 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     1381 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1441 ctctcggcat ggacgagctg tacaagtaat aatactagag ccaggcatca aataaaacga
     1501 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1561 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//