LOCUS       BBa_I0406               1747 bp    DNA     linear       22-Dec-2024
DEFINITION  R0040.B0034.C0079.E0420
ACCESSION   BBa_I0406
VERSION     BBa_I0406.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..797
                     /label=lasR
     misc_feature    798..830
                     /label=LVA
     misc_feature    560..560
                     /label=G
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0079
     misc_feature    874..877
                     /label=conserved
     RBS             870..881
                     /label=BBa_B0034
     CDS             888..1610
                     /label=BBa_E0020
     stem_loop       1630..1673
                     /label=stem_loop
     misc_feature    1619..1698
                     /label=BBa_B0010
     terminator      1619..1698
                     /label=BBa_B0010
     stem_loop       1714..1733
                     /label=T7 TE
     misc_feature    1740..1740
                     /label=stop
     polyA_site      1734..1747
                     /label=polya
     misc_feature    1707..1747
                     /label=BBa_B0012
     terminator      1707..1747
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggccttgg ttgacggttt tcttgagctg gaacgctcaa
      121 gtggaaaatt ggagtggagc gccatcctcc agaagatggc gagcgacctt ggattctcga
      181 agatcctgtt cggcctgttg cctaaggaca gccaggacta cgagaacgcc ttcatcgtcg
      241 gcaactaccc ggccgcctgg cgcgagcatt acgaccgggc tggctacgcg cgggtcgacc
      301 cgacggtcag tcactgtacc cagagcgtac tgccgatttt ctgggaaccg tccatctacc
      361 agacgcgaaa gcagcacgag ttcttcgagg aagcctcggc cgccggcctg gtgtatgggc
      421 tgaccatgcc gctgcatggt gctcgcggcg aactcggcgc gctgagcctc agcgtggaag
      481 cggaaaaccg ggccgaggcc aaccgtttca tagagtcggt cctgccgacc ctgtggatgc
      541 tcaaggacta cgcactgcaa agcggtgccg gactggcctt cgaacatccg gtcagcaaac
      601 cggtggttct gaccagccgg gagaaggaag tgttgcagtg gtgcgccatc ggcaagacca
      661 gttgggagat atcggttatc tgcaactgct cggaagccaa tgtgaacttc catatgggaa
      721 atattcggcg gaagttcggt gtgacctccc gccgcgtagc ggccattatg gccgttaatt
      781 tgggtcttat tactctcgct gcaaacgacg aaaactacgc tttagtagct taataacact
      841 gatagtgcta gtgtagatca ctactagaga aagaggagaa atactagatg gtgagcaagg
      901 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg
      961 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1021 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     1081 tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     1141 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1201 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1261 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1321 actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
     1381 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     1441 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     1501 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1561 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaataa tactagagcc
     1621 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1681 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1741 gtttata
//