##gff-version 3
##sequence-region BBa_I0406 1 1747
BBa_I0406	.	ribosome_entry_site	870	881	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I0406	.	conserved_region	874	877	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I0406	.	CDS	888	1610	.	+	0	ID=BBa_E0020;Name=BBa_E0020
BBa_I0406	.	terminator	1707	1747	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I0406	.	stem_loop	1714	1733	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I0406	.	stop_codon	1740	1740	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I0406	.	polyA_site	1734	1747	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I0406	.	engineered_region	1707	1747	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I0406	.	terminator	1619	1698	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I0406	.	stem_loop	1630	1673	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I0406	.	engineered_region	1619	1698	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I0406	.	CDS	81	836	.	+	0	ID=BBa_C0079;Name=BBa_C0079
BBa_I0406	.	tag	798	830	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_I0406	.	sequence_alteration	560	560	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_I0406	.	engineered_tag	837	861	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_I0406	.	CDS	81	797	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_I0406	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I0406	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I0406	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I0406	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I0406	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I0406	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I0406	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I0406	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I0406
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatg
gcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcg
tcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactg
tacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcc
tcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcg
tggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgc
actgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtg
ttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttcc
atatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttat
tactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagag
aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacg
gcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagtt
catctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagc
cgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatct
tcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagct
gaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctat
atcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagc
tcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcaccca
gtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcact
ctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttata