LOCUS       BBa_I0407               1633 bp    DNA     linear       22-Dec-2024
DEFINITION  R0040.B0034.C0078.E0420
ACCESSION   BBa_I0407
VERSION     BBa_I0407.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..683
                     /label=LasI
     misc_feature    684..716
                     /label=LVA
     misc_feature    723..747
                     /label=Help:Barcodes
     CDS             81..722
                     /label=BBa_C0078
     misc_feature    760..763
                     /label=conserved
     RBS             756..767
                     /label=BBa_B0034
     CDS             774..1496
                     /label=BBa_E0020
     stem_loop       1516..1559
                     /label=stem_loop
     misc_feature    1505..1584
                     /label=BBa_B0010
     terminator      1505..1584
                     /label=BBa_B0010
     stem_loop       1600..1619
                     /label=T7 TE
     misc_feature    1626..1626
                     /label=stop
     polyA_site      1620..1633
                     /label=polya
     misc_feature    1593..1633
                     /label=BBa_B0012
     terminator      1593..1633
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatcgttc agatcggtcg tcgtgaagag ttcgacaaaa
      121 aactgctggg tgaaatgcac aaactgcgtg ctcaggtttt caaagaacgt aaaggttggg
      181 acgtttccgt tatcgacgaa atggaaatcg acggttacga cgctctgtcc ccgtactaca
      241 tgctgatcca ggaagacacc ccggaagctc aggttttcgg ttgctggcgt atcttcgaca
      301 ccaccggtcc gtacatgctg aaaaacacct tcccggaact gctgcacggt aaagaagctc
      361 cgtgctcccc gcacatctgg gaactgtccc gtttcgctat caactccggt cagaaaggtt
      421 ccctgggttt ctccgactgc accctggaag ctatgcgtgc tctggctcgt tactccttgc
      481 agaacgacat ccagaccctg gttaccgtta ccaccgttgg tgttgaaaaa atgatgatcc
      541 gtgctggtct ggacgtttcc cgtttcggtc cgcacctgaa aatcggtatc gaacgtgctg
      601 ttgctctgcg tatcgaactg aacgctaaaa cccagatcgc tctgtacggt ggtgttctgg
      661 ttgaacagcg tctggctgtt tccgctgcta acgacgaaaa ctacgctctg gttgcttaat
      721 aactctgata gtgctagtgt agatctctac tagagaaaga ggagaaatac tagatggtga
      781 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      841 tgaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      901 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      961 ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     1021 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1081 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1141 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1201 agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     1261 aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1321 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1381 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1441 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taataatact
     1501 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1561 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1621 ttctgcgttt ata
//