LOCUS       BBa_I0409               1693 bp    DNA     linear       16-Feb-2025
DEFINITION  R0040.B0034.C0076.E0420
ACCESSION   BBa_I0409
VERSION     BBa_I0409.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..743
                     /label=CinI
     misc_feature    744..776
                     /label=LVA
     misc_feature    783..807
                     /label=Help:Barcodes
     CDS             81..782
                     /label=BBa_C0076
     misc_feature    820..823
                     /label=conserved
     RBS             816..827
                     /label=BBa_B0034
     CDS             834..1556
                     /label=BBa_E0020
     stem_loop       1576..1619
                     /label=stem_loop
     misc_feature    1565..1644
                     /label=BBa_B0010
     terminator      1565..1644
                     /label=BBa_B0010
     stem_loop       1660..1679
                     /label=T7 TE
     misc_feature    1686..1686
                     /label=stop
     polyA_site      1680..1693
                     /label=polya
     misc_feature    1653..1693
                     /label=BBa_B0012
     terminator      1653..1693
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgttcgtta tcatccaggc tcacgaatac cagaaatacg
      121 ctgctgttct ggaccagatg ttccgtctgc gtaaaaaagt tttcgctgac accctgtgct
      181 gggacgttcc ggttatcggt ccgtacgaac gtgactccta cgactccctg gctccggctt
      241 acctggtttg gtgcaacgac tcccgtaccc gtctgtacgg tggtatgcgt ctgatgccga
      301 ccaccggtcc gaccctgctg tacgacgttt tccgtgaaac cttcccggac gctgctgacc
      361 tgatcgctcc gggtatctgg gaaggtaccc gtatgtgcat cgacgaagaa gctatcgcta
      421 aagacttccc ggaaatcgac gctggtcgtg ctttctccat gatgctgctg gctctgtgcg
      481 aatgcgctct ggaccacggt atccacacca tgatctccaa ctacgaaccg tacctgaaac
      541 gtgtttacaa acgtgctggt gctgaagttg aagaactggg tcgtgctgac ggttacggta
      601 aatacccggt ttgctgcggt gctttcgaag tttccgaccg tgttctgcgt aaaatgcgtg
      661 ctgctctggg tctgaccctg ccgctgtacg ttcgtcacgt tccggctcgt tccgttgtta
      721 cccagttcct ggaaatggct gctgctgcta acgacgaaaa ctacgctctg gttgcttaat
      781 aaccctgata gtgctagtgt agatccctac tagagaaaga ggagaaatac tagatggtga
      841 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      901 tgaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      961 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1021 ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     1081 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1141 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1201 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1261 agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     1321 aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1381 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1441 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1501 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taataatact
     1561 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1621 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1681 ttctgcgttt ata
//