LOCUS BBa_I0414 941 bp DNA linear 22-Nov-2024 DEFINITION RBS Test R0040.B0032.E0030.B0015 RBS Test ACCESSION BBa_I0414 VERSION BBa_I0414.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..75 /label=RBS-3\Weak RBS 69..72 /label=RBS misc_feature 63..75 /label=BBa_B0032 RBS 63..75 /label=BBa_B0032 CDS 82..804 /label=BBa_E0030 stem_loop 824..867 /label=stem_loop misc_feature 813..892 /label=BBa_B0010 terminator 813..892 /label=BBa_B0010 stem_loop 908..927 /label=T7 TE misc_feature 934..934 /label=stop polyA_site 928..941 /label=polya misc_feature 901..941 /label=BBa_B0012 terminator 901..941 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agtcacacag gaaagtacta gatggtgagc aagggcgagg agctgttcac cggggtggtg 121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 241 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc 301 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac 361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 661 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac 721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 781 atggacgagc tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca 841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 901 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //