LOCUS       BBa_I0424               2700 bp    DNA     linear       22-Nov-2024
DEFINITION  I0404.I6101
ACCESSION   BBa_I0424
VERSION     BBa_I0424.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..803
                     /label=rhlR
     misc_feature    804..836
                     /label=LVA
     misc_feature    843..867
                     /label=Help:Barcodes
     CDS             81..842
                     /label=BBa_C0071
     misc_feature    880..883
                     /label=conserved
     RBS             876..887
                     /label=BBa_B0034
     CDS             894..1616
                     /label=BBa_E0020
     stem_loop       1636..1679
                     /label=stem_loop
     misc_feature    1625..1704
                     /label=BBa_B0010
     terminator      1625..1704
                     /label=BBa_B0010
     stem_loop       1720..1739
                     /label=T7 TE
     misc_feature    1746..1746
                     /label=stop
     polyA_site      1740..1753
                     /label=polya
     misc_feature    1713..1753
                     /label=BBa_B0012
     terminator      1713..1753
                     /label=BBa_B0012
     misc_feature    1762..1781
                     /label=RhlR
     promoter        1780..1785
                     /label=-35
     promoter        1803..1808
                     /label=-10
     misc_feature    1814..1814
                     /label=start
     promoter        1762..1814
                     /label=BBa_R0071
     misc_feature    1827..1830
                     /label=conserved
     RBS             1823..1834
                     /label=BBa_B0034
     CDS             1841..2563
                     /label=BBa_E0030
     stem_loop       2583..2626
                     /label=stem_loop
     misc_feature    2572..2651
                     /label=BBa_B0010
     terminator      2572..2651
                     /label=BBa_B0010
     stem_loop       2667..2686
                     /label=T7 TE
     misc_feature    2693..2693
                     /label=stop
     polyA_site      2687..2700
                     /label=polya
     misc_feature    2660..2700
                     /label=BBa_B0012
     terminator      2660..2700
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaggaatg acggaggctt tttgctgtgg tgggacggtt
      121 tgcgtagcga gatgcagccg atccacgaca gccagggcgt gttcgccgtc ctggaaaagg
      181 aagtgcggcg cctgggcttc gattactacg cctatggcgt gcgccacacg attcccttca
      241 cccggccgaa gaccgaggtc catggcacct atcccaaggc ctggctggag cgataccaga
      301 tgcagaacta cggggccgtg gatccggcga tcctcaacgg cctgcgctcc tcggaaatgg
      361 tggtctggag cgacagcctg ttcgaccaga gccggatgct ctggaacgag gctcgcgatt
      421 ggggcctctg tgtcggcgcg accttgccga tccgcgcgcc gaacaatttg ctcagcgtgc
      481 tttccgtggc gcgcgaccag cagaacatct ccagcttcga gcgcgaggaa atccgcctgc
      541 ggctgcgttg catgatcgag ttgctgaccc agaagctgac cgacctggag catccgatgc
      601 tgatgtccaa cccggtctgc ctgagccatc gcgaacgcga gatcctgcaa tggaccgccg
      661 acggcaagag ttccggggaa atcgccatca tcctgagcat ctccgagagc acggtgaact
      721 tccaccacaa gaacatccag aagaagttcg acgcgccgaa caagacgctg gctgccgcct
      781 acgccgcggc gctgggtctc atcgctgcaa acgacgaaaa ctacgcttta gtagcttaat
      841 aacactgata gtgctagtgt agatcactac tagagaaaga ggagaaatac tagatggtga
      901 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      961 tgaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1021 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1081 ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     1141 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1201 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1261 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1321 agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     1381 aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1441 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1501 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1561 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taataatact
     1621 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1681 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1741 ttctgcgttt atatactaga gtcctgtgaa atctggcagt taccgttagc tttcgaattg
     1801 gctaaaaagt gttctactag agaaagagga gaaatactag atggtgagca agggcgagga
     1861 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1921 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1981 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta
     2041 cggcctgcaa tgcttcgccc gctaccccga ccacatgaag ctgcacgact tcttcaagtc
     2101 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2161 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2221 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2281 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2341 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2401 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc
     2461 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2521 cgccgggatc actctcggca tggacgagct gtacaagtaa taatactaga gccaggcatc
     2581 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2641 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//