LOCUS       BBa_I0428               2872 bp    DNA     linear       22-Nov-2024
DEFINITION  I0408.I6106
ACCESSION   BBa_I0428
VERSION     BBa_I0428.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..803
                     /label=cinR
     misc_feature    804..836
                     /label=LVA
     misc_feature    843..867
                     /label=Help:Barcodes
     CDS             81..842
                     /label=BBa_C0077
     misc_feature    880..883
                     /label=conserved
     RBS             876..887
                     /label=BBa_B0034
     CDS             894..1616
                     /label=BBa_E0020
     stem_loop       1636..1679
                     /label=stem_loop
     misc_feature    1625..1704
                     /label=BBa_B0010
     terminator      1625..1704
                     /label=BBa_B0010
     stem_loop       1720..1739
                     /label=T7 TE
     misc_feature    1746..1746
                     /label=stop
     polyA_site      1740..1753
                     /label=polya
     misc_feature    1713..1753
                     /label=BBa_B0012
     terminator      1713..1753
                     /label=BBa_B0012
     misc_feature    1762..1981
                     /label=unidentified/uncharacterized CinR dependent
     stem_loop       1796..1835
                     /label=stem_loop
     stem_loop       1852..1897
                     /label=stem_loop
     promoter        1762..1986
                     /label=BBa_R0078
     misc_feature    1999..2002
                     /label=conserved
     RBS             1995..2006
                     /label=BBa_B0034
     CDS             2013..2735
                     /label=BBa_E0030
     stem_loop       2755..2798
                     /label=stem_loop
     misc_feature    2744..2823
                     /label=BBa_B0010
     terminator      2744..2823
                     /label=BBa_B0010
     stem_loop       2839..2858
                     /label=T7 TE
     misc_feature    2865..2865
                     /label=stop
     polyA_site      2859..2872
                     /label=polya
     misc_feature    2832..2872
                     /label=BBa_B0012
     terminator      2832..2872
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgattgaga atacctatag cgaaaagttc gagtccgcgt
      121 tcgaacagat caaggcggcg gccaacgtgg atgccgccat ccgtattctc caggcggaat
      181 ataacctcga tttcgtcacc taccatctcg cccagacgat cgcgagcaag atcgattcgc
      241 ccttcgtgcg caccacctat ccggatgcct gggtttcccg ctacctcctc aacagctatg
      301 tgaaggtcga tccgatcgtc aagcagggct tcgaacgcca gctgcccttc gactggagcg
      361 aggtcgaacc gacgccggag gcctatgcca tgctggtcga cgcccagaaa cacggcatcg
      421 gtggcaatgg ctactccatc cccgtcgccg acaaggcgca gcgccgcgcc ctgctgtcgc
      481 tgaatgcccg tataccggcc gacgaatgga ccgagctcgt gcgccgctgc cgcaacgagt
      541 ggatcgagat cgcccatctg atccaccgca aggccgtcta tgagctgcat ggcgaaaacg
      601 atccggtgcc ggcattgtcg ccgcgcgaga tcgagtgtct gcactggacc gccctcggca
      661 aggattacaa ggatatttcg gtcatcctgg gcatatcaga gcataccaca cgcgattacc
      721 tgaagaccgc ccgcttcaag ctcggctgcg ccacgatctc ggccgccgcg tcgcgggctg
      781 ttcaattgcg catcatcaat cccgctgcaa acgacgaaaa ctacgcttta gtagcttaat
      841 aacgctgata gtgctagtgt agatcgctac tagagaaaga ggagaaatac tagatggtga
      901 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      961 tgaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1021 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1081 ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     1141 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1201 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1261 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1321 agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     1381 aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1441 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1501 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1561 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taataatact
     1621 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1681 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1741 ttctgcgttt atatactaga gccctttgtg cgtccaaacg gacgcacggc gctctaaagc
     1801 gggtcgcgat ctttcagatt cgctcctcgc gctttcagtc tttgttttgg cgcatgtcgt
     1861 tatcgcaaaa ccgctgcaca cttttgcgcg acatgctctg atccccctca tctggggggg
     1921 cctatctgag ggaatttccg atccggctcg cctgaaccat tctgctttcc acgaacttga
     1981 aaacgctact agagaaagag gagaaatact agatggtgag caagggcgag gagctgttca
     2041 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     2101 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     2161 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc
     2221 aatgcttcgc ccgctacccc gaccacatga agctgcacga cttcttcaag tccgccatgc
     2281 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     2341 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     2401 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     2461 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     2521 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     2581 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca
     2641 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     2701 tcactctcgg catggacgag ctgtacaagt aataatacta gagccaggca tcaaataaaa
     2761 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2821 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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