LOCUS       BBa_I0429               2858 bp    DNA     linear       22-Dec-2024
DEFINITION  I0408.I6116
ACCESSION   BBa_I0429
VERSION     BBa_I0429.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..803
                     /label=cinR
     misc_feature    804..836
                     /label=LVA
     misc_feature    843..867
                     /label=Help:Barcodes
     CDS             81..842
                     /label=BBa_C0077
     misc_feature    880..883
                     /label=conserved
     RBS             876..887
                     /label=BBa_B0034
     CDS             894..1616
                     /label=BBa_E0020
     stem_loop       1636..1679
                     /label=stem_loop
     misc_feature    1625..1704
                     /label=BBa_B0010
     terminator      1625..1704
                     /label=BBa_B0010
     stem_loop       1720..1739
                     /label=T7 TE
     misc_feature    1746..1746
                     /label=stop
     polyA_site      1740..1753
                     /label=polya
     misc_feature    1713..1753
                     /label=BBa_B0012
     terminator      1713..1753
                     /label=BBa_B0012
     RBS             1987..1992
                     /label=embedded RBS
     stem_loop       1796..1835
                     /label=stem_loop
     stem_loop       1852..1897
                     /label=stem_loop
     promoter        1762..1981
                     /label=unidentified/uncharacterized CinR dependent
     promoter        1762..1992
                     /label=BBa_R0077
     CDS             1999..2721
                     /label=BBa_E0030
     stem_loop       2741..2784
                     /label=stem_loop
     misc_feature    2730..2809
                     /label=BBa_B0010
     terminator      2730..2809
                     /label=BBa_B0010
     stem_loop       2825..2844
                     /label=T7 TE
     misc_feature    2851..2851
                     /label=stop
     polyA_site      2845..2858
                     /label=polya
     misc_feature    2818..2858
                     /label=BBa_B0012
     terminator      2818..2858
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgattgaga atacctatag cgaaaagttc gagtccgcgt
      121 tcgaacagat caaggcggcg gccaacgtgg atgccgccat ccgtattctc caggcggaat
      181 ataacctcga tttcgtcacc taccatctcg cccagacgat cgcgagcaag atcgattcgc
      241 ccttcgtgcg caccacctat ccggatgcct gggtttcccg ctacctcctc aacagctatg
      301 tgaaggtcga tccgatcgtc aagcagggct tcgaacgcca gctgcccttc gactggagcg
      361 aggtcgaacc gacgccggag gcctatgcca tgctggtcga cgcccagaaa cacggcatcg
      421 gtggcaatgg ctactccatc cccgtcgccg acaaggcgca gcgccgcgcc ctgctgtcgc
      481 tgaatgcccg tataccggcc gacgaatgga ccgagctcgt gcgccgctgc cgcaacgagt
      541 ggatcgagat cgcccatctg atccaccgca aggccgtcta tgagctgcat ggcgaaaacg
      601 atccggtgcc ggcattgtcg ccgcgcgaga tcgagtgtct gcactggacc gccctcggca
      661 aggattacaa ggatatttcg gtcatcctgg gcatatcaga gcataccaca cgcgattacc
      721 tgaagaccgc ccgcttcaag ctcggctgcg ccacgatctc ggccgccgcg tcgcgggctg
      781 ttcaattgcg catcatcaat cccgctgcaa acgacgaaaa ctacgcttta gtagcttaat
      841 aacgctgata gtgctagtgt agatcgctac tagagaaaga ggagaaatac tagatggtga
      901 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      961 tgaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1021 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1081 ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     1141 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     1201 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1261 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1321 agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     1381 aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1441 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1501 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1561 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taataatact
     1621 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1681 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1741 ttctgcgttt atatactaga gccctttgtg cgtccaaacg gacgcacggc gctctaaagc
     1801 gggtcgcgat ctttcagatt cgctcctcgc gctttcagtc tttgttttgg cgcatgtcgt
     1861 tatcgcaaaa ccgctgcaca cttttgcgcg acatgctctg atccccctca tctggggggg
     1921 cctatctgag ggaatttccg atccggctcg cctgaaccat tctgctttcc acgaacttga
     1981 aaacgctgga ggtactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2041 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     2101 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2161 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc
     2221 taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     2281 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2341 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2401 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     2461 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     2521 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2581 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     2641 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     2701 gacgagctgt acaagtaata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     2761 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     2821 cactggctca ccttcgggtg ggcctttctg cgtttata
//