LOCUS       BBa_I1010                834 bp    DNA     linear       25-Dec-2024
DEFINITION  cI(1) fused to tetR promoter
ACCESSION   BBa_I1010
VERSION     BBa_I1010.1  
FEATURES             Location/Qualifiers
     CDS             82..834
                     /label=cI
     misc_feature    82..84
                     /label=start
     RBS             72..77
                     /label=rbs
     CDS             85..157
                     /label=Modified cI cds
     misc_feature    1..19
                     /label=TetR 1
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     misc_feature    55..55
                     /label=start
     promoter        1..54
                     /label=tetR
     misc_feature    796..834
                     /label=SsrA
     misc_feature    829..834
                     /label=2
     misc_feature    55..157
                     /label=Complementary to Antisense mRNA
     misc_feature    85..87
                     /label=added codon (Cys)
     misc_feature    1..834
                     /label=BBa_I1010
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacgcgaaa
       61 aatcaataat cagacaacaa gatgtgctcg acaaaaaaga aaccattaac acaagagcag
      121 cttgaggacg cacgtcgcct taaagcaatt tatgaaaaaa agaaaaatga acttggctta
      181 tcccaggaat ctgtcgcaga caagatgggg atggggcagt caggcgttgg tgctttattt
      241 aatggcatca atgcattaaa tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt
      301 agcgttgaag aatttagccc ttcaatcgcc agagaaatct acgagatgta tgaagcggtt
      361 agtatgcagc cgtcacttag aagtgagtat gagtaccctg ttttttctca tgttcaggca
      421 gggatgttct cacctgagct tagaaccttt accaaaggtg atgcggagag atgggtaagc
      481 acaaccaaaa aagccagtga ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc
      541 gcaccaacag gctccaagcc aagctttcct gacggaatgt taattctcgt tgaccctgag
      601 caggctgttg agccaggtga tttctgcata gccagacttg ggggtgatga gtttaccttc
      661 aagaaactga tcagggatag cggtcaggtg tttttacaac cactaaaccc acagtaccca
      721 atgatcccat gcaatgagag ttgttccgtt gtggggaaag ttatcgctag tcagtggcct
      781 gaagagacgt ttggcgctgc aaacgacgaa aactacgctt tagtagctta ataa
//