##gff-version 3
##sequence-region BBa_I1010 1 834
BBa_I1010	.	start_codon	82	84	.	+	0	ID=annotation1781;Name=start
BBa_I1010	.	sequence_alteration	85	87	.	+	0	ID=annotation1794;Name=added codon (Cys)
BBa_I1010	.	promoter	43	48	.	+	0	ID=annotation1787;Name=-10
BBa_I1010	.	start_codon	55	55	.	+	0	ID=annotation1788;Name=start
BBa_I1010	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1784;Name=TetR 1
BBa_I1010	.	promoter	20	25	.	+	0	ID=annotation1785;Name=-35
BBa_I1010	.	promoter	1	54	.	+	0	ID=annotation1789;Name=tetR
BBa_I1010	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1786;Name=TetR 2
BBa_I1010	.	engineered_region	1	834	.	+	0	ID=annotation7042;Name=BBa_I1010
BBa_I1010	.	CDS	82	834	.	+	0	ID=annotation1780;Name=cI
BBa_I1010	.	tag	796	834	.	+	0	ID=annotation1790;Name=SsrA
BBa_I1010	.	ribosome_entry_site	72	77	.	+	0	ID=annotation1782;Name=rbs
BBa_I1010	.	sequence_feature	55	157	.	+	0	ID=annotation1793;Name=Complementary to Antisense mRNA
BBa_I1010	.	CDS	85	157	.	+	0	ID=annotation1783;Name=Modified cI cds
BBa_I1010	.	stop_codon	829	834	.	+	0	ID=annotation1791;Name=2
>BBa_I1010
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacgcgaaaaatcaataatcagacaaca
agatgtgctcgacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaa
aaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgcttta
tttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaattta
gcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagta
ccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgg
gtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggct
ccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagc
cagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaaccca
cagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgt
ttggcgctgcaaacgacgaaaactacgctttagtagcttaataa