BBa_I1011
1
BBa_I1011
CI(1) "KISS" asRNA without promoter
2003-01-31T12:00:00Z
2015-08-31T04:07:29Z
Custom design.
Region which serves as basis for transcription of asRNA that binds to and inhibits <bb_part>BBa_I1010</bb_part>'s mRNA transcript. Has tetR promoter <bb_part>BBa_R0040</bb_part>. Part of the XOR gate comprised of <bb_part>BBa_I1010</bb_part> and <bb_part>BBa_I1020</bb_part>, their corresponding asRNA coding sequences (<bb_part>BBa_I1011</bb_part> and <bb_part>BBa_I1021</bb_part>), and the LacO-1 and TetR <bb_part>BBa_R0040</bb_part> promoters.
false
false
_1_
0
24
7
It's complicated
false
References (unparsed) here: <p>Coleman, J., et al. <em>Nature</em>. (1985) 315, 601-3.<P> Pestka, S., et al. <em>Proc. Natl. Acad. Sci. USA</em> (1984) 81, 7525-28.<P> References (unparsed) here: <p>Coleman, J., et al. <em>Nature</em>. (1985) 315, 601-3.<P> Pestka, S., et al. <em>Proc. Natl. Acad. Sci. USA</em> (1984) 81, 7525-28.<P>Complementary to beginning of <bb_part>BBa_I1010</bb_part> transcript covering RBS, start codon, and 73 bp into coding sequence. No designed secondary structure. <blockquote> <B>Anti-sense</B></P> <P>The success of this system clearly rests on the ability to effectively and specifically target mRNA transcripts for degradation using anti-sense RNA. While many papers, articles, and books have been written on the subject, there are no consensus anti-sense building strategies presented. We thus chose to implement three different types of antisense inhibition: KISS, micRNA, and IS10. In the description that follows, the following nomenclature will be used:</P> <P><em>target</em>- the mRNA transcript that we wish to inhibit.</P> <P><em>anti-sense</em>- the anti-sense molecule which will bind and inhibit <em>target</em>. </P> <blockquote> <P><em><strong>KISS (Keep it SImple, Silly)</strong></em></P> <img src="http://biobricks.ai.mit.edu/IAP_Projects/YoungPower/as_KISS.gif"> <P>The simplest of the three methods, this type relies on a single-stranded linear 103 bp anti-sense that is specific to the target of interest. In addition, the first 76 base pairs of the cI region of BBa_I1010 have been codon-modified to give a different sequence that codes for the same cI protein (See BBa_I1030 and I1040). </P> <P>BBa_I1011 contains the reverse complement of the RBS, start codon, and 76 bp region for BBa_I1010. Thus, if both BBa_I1010 and BBa_I1011 are transcribed, the transcripts will bind to each other and BBa_I1010 will not be translated. </P> <P>Note that BBa_I1010 already contains a regulatory region, RBS, and coding region (a terminator must be added), while BBa_I1011 does not - thus, when using this component, the appropriate regulatory region, RBS, and terminator must be added to this part.</P> </blockquote></blockquote><P> Incompatible with systems containing <bb_part>BBa_I1012</bb_part>, <bb_part>BBa_I1013</bb_part>. <br>Compatible with <bb_part>BBa_I1020</bb_part>, <bb_part>BBa_I1021</bb_part>, <bb_part>BBa_I1022</bb_part>, <bb_part>BBa_I1023</bb_part>.
false
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1801
1
added codon (Cys)
range1801
1
71
73
annotation1798
1
Reverse Complement RBS
range1798
1
81
86
annotation1797
1
Reverse Complement to cI mRNA
range1797
1
1
103
annotation7043
1
BBa_I1011
range7043
1
1
103
annotation1799
1
reverse complement to cI cds
range1799
1
1
73
annotation1800
1
start
range1800
1
74
76
BBa_I1011_sequence
1
tttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtcgagcacatcttgttgtctgattattgatttttcgc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z