BBa_I1011 1 BBa_I1011 CI(1) &quot;KISS&quot; asRNA without promoter 2003-01-31T12:00:00Z 2015-08-31T04:07:29Z Custom design. Region which serves as basis for transcription of asRNA that binds to and inhibits <bb_part>BBa_I1010</bb_part>'s mRNA transcript. Has tetR promoter <bb_part>BBa_R0040</bb_part>. Part of the XOR gate comprised of <bb_part>BBa_I1010</bb_part> and <bb_part>BBa_I1020</bb_part>, their corresponding asRNA coding sequences (<bb_part>BBa_I1011</bb_part> and <bb_part>BBa_I1021</bb_part>), and the LacO-1 and TetR <bb_part>BBa_R0040</bb_part> promoters. false false _1_ 0 24 7 It's complicated false References (unparsed) here: <p>Coleman, J., et al. <em>Nature</em>. (1985) 315, 601-3.<P> Pestka, S., et al. <em>Proc. Natl. Acad. Sci. USA</em> (1984) 81, 7525-28.<P> References (unparsed) here: <p>Coleman, J., et al. <em>Nature</em>. (1985) 315, 601-3.<P> Pestka, S., et al. <em>Proc. Natl. Acad. Sci. USA</em> (1984) 81, 7525-28.<P>Complementary to beginning of <bb_part>BBa_I1010</bb_part> transcript covering RBS, start codon, and 73 bp into coding sequence. No designed secondary structure. <blockquote> <B>Anti-sense</B></P> <P>The success of this system clearly rests on the ability to effectively and specifically target mRNA transcripts for degradation using anti-sense RNA. While many papers, articles, and books have been written on the subject, there are no consensus anti-sense building strategies presented. We thus chose to implement three different types of antisense inhibition: KISS, micRNA, and IS10. In the description that follows, the following nomenclature will be used:</P> <P><em>target</em>- the mRNA transcript that we wish to inhibit.</P> <P><em>anti-sense</em>- the anti-sense molecule which will bind and inhibit <em>target</em>. </P> <blockquote> <P><em><strong>KISS (Keep it SImple, Silly)</strong></em></P> <img src="http://biobricks.ai.mit.edu/IAP_Projects/YoungPower/as_KISS.gif"> <P>The simplest of the three methods, this type relies on a single-stranded linear 103 bp anti-sense that is specific to the target of interest. In addition, the first 76 base pairs of the cI region of BBa_I1010 have been codon-modified to give a different sequence that codes for the same cI protein (See BBa_I1030 and I1040). </P> <P>BBa_I1011 contains the reverse complement of the RBS, start codon, and 76 bp region for BBa_I1010. Thus, if both BBa_I1010 and BBa_I1011 are transcribed, the transcripts will bind to each other and BBa_I1010 will not be translated. </P> <P>Note that BBa_I1010 already contains a regulatory region, RBS, and coding region (a terminator must be added), while BBa_I1011 does not - thus, when using this component, the appropriate regulatory region, RBS, and terminator must be added to this part.</P> </blockquote></blockquote><P> Incompatible with systems containing <bb_part>BBa_I1012</bb_part>, <bb_part>BBa_I1013</bb_part>. <br>Compatible with <bb_part>BBa_I1020</bb_part>, <bb_part>BBa_I1021</bb_part>, <bb_part>BBa_I1022</bb_part>, <bb_part>BBa_I1023</bb_part>. false June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1801 1 added codon (Cys) range1801 1 71 73 annotation1800 1 start range1800 1 74 76 annotation1797 1 Reverse Complement to cI mRNA range1797 1 1 103 annotation1799 1 reverse complement to cI cds range1799 1 1 73 annotation7043 1 BBa_I1011 range7043 1 1 103 annotation1798 1 Reverse Complement RBS range1798 1 81 86 BBa_I1011_sequence 1 tttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtcgagcacatcttgttgtctgattattgatttttcgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z