BBa_I1020
1
BBa_I1020
Custom cI(2) Protein w/LacO-1 Promoter
2003-01-31T12:00:00Z
2015-08-31T04:07:29Z
Modified from BioBricks <bb_part>BBa_R0011</bb_part>, and <bb_part>BBa_C0051</bb_part>.
Coding sequence for modified cI protein with LacO-1 promoter for antisense IS10 mRNA regulation.
false
false
_1_
0
24
7
It's complicated
false
<P> <P> <bb_part>BBa_I1020</bb_part> Custom cI Protein with LacO-1 Promoter is based on BioBricks <bb_part>BBa_C0050</bb_part> and <bb_part>BBa_R0011</bb_part>. It is designed such that <bb_part>BBa_I1023</bb_part> can be used to interfere with the <bb_part>BBa_1020</bb_part> transcript by anti-sense mRNA binding, in the IS10 method shown below. <bb_part>BBa_I1020</bb_part> and <bb_part>BBa_I1023</bb_part> can act indepently of related biobrick parts <bb_part>BBa_1010</bb_part>, <bb_part>BBa_1011</bb_part>, <bb_part>BBa_1012</bb_part>, <bb_part>BBa_I1030</bb_part>, <bb_part>BBa_I1031</bb_part>, <bb_part>BBa_I1032</bb_part>, <bb_part>BBa_I1041</bb_part>, <bb_part>BBa_I1042</bb_part>, <bb_part>BBa_I1060</bb_part>, <bb_part>BBa_I1063</bb_part> and <bb_part>BBa_1013</bb_part>. <P>Can be used with all parts of the BBa_I system. It is inhibited by BBa_I1023. Incompatible with systems containing cI (wild type)
false
Samantha Sutton, Neelaksh Varshney, Daniel Shen, Grace Kenney
annotation1830
1
LacO-1 region
range1830
1
1
20
annotation1826
1
-10
range1826
1
44
49
annotation1833
1
HH117a mutation specific to I1020 and I1023
range1833
1
58
58
annotation1828
1
SsrA
range1828
1
797
835
annotation1823
1
start
range1823
1
83
85
annotation1827
1
Complementary to Antisense mRNA I1023
range1827
1
56
90
annotation1820
1
stem_loop
range1820
1
56
85
annotation1829
1
2
range1829
1
830
835
annotation1832
1
start
range1832
1
56
56
annotation1831
1
LacO-1
range1831
1
27
43
annotation1834
1
C added
range1834
1
86
88
annotation7046
1
BBa_I1020
range7046
1
1
835
annotation1824
1
cI lambda
range1824
1
83
835
annotation1821
1
rbs
range1821
1
73
78
annotation1825
1
-35
range1825
1
21
26
annotation1835
1
Homology to I1023
range1835
1
89
90
BBa_I1020_sequence
1
ataaatgtgagcggataacattgacattgtgagcggataacaagatactgagcacgccaaaaatcaataatcagacaacaagatgtgcagtactaagaaaaagcctttgactcaggaacaactcgaagatgcccgccgtctcaaggccatctacgagaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z