BBa_I1020 1 BBa_I1020 Custom cI(2) Protein w/LacO-1 Promoter 2003-01-31T12:00:00Z 2015-08-31T04:07:29Z Modified from BioBricks <bb_part>BBa_R0011</bb_part>, and <bb_part>BBa_C0051</bb_part>. Coding sequence for modified cI protein with LacO-1 promoter for antisense IS10 mRNA regulation. false false _1_ 0 24 7 It's complicated false <P> <P> <bb_part>BBa_I1020</bb_part> Custom cI Protein with LacO-1 Promoter is based on BioBricks <bb_part>BBa_C0050</bb_part> and <bb_part>BBa_R0011</bb_part>. It is designed such that <bb_part>BBa_I1023</bb_part> can be used to interfere with the <bb_part>BBa_1020</bb_part> transcript by anti-sense mRNA binding, in the IS10 method shown below. <bb_part>BBa_I1020</bb_part> and <bb_part>BBa_I1023</bb_part> can act indepently of related biobrick parts <bb_part>BBa_1010</bb_part>, <bb_part>BBa_1011</bb_part>, <bb_part>BBa_1012</bb_part>, <bb_part>BBa_I1030</bb_part>, <bb_part>BBa_I1031</bb_part>, <bb_part>BBa_I1032</bb_part>, <bb_part>BBa_I1041</bb_part>, <bb_part>BBa_I1042</bb_part>, <bb_part>BBa_I1060</bb_part>, <bb_part>BBa_I1063</bb_part> and <bb_part>BBa_1013</bb_part>. <P>Can be used with all parts of the BBa_I system. It is inhibited by BBa_I1023. Incompatible with systems containing cI (wild type) false Samantha Sutton, Neelaksh Varshney, Daniel Shen, Grace Kenney annotation1831 1 LacO-1 range1831 1 27 43 annotation1820 1 stem_loop range1820 1 56 85 annotation1832 1 start range1832 1 56 56 annotation1826 1 -10 range1826 1 44 49 annotation1824 1 cI lambda range1824 1 83 835 annotation7046 1 BBa_I1020 range7046 1 1 835 annotation1829 1 2 range1829 1 830 835 annotation1821 1 rbs range1821 1 73 78 annotation1823 1 start range1823 1 83 85 annotation1835 1 Homology to I1023 range1835 1 89 90 annotation1834 1 C added range1834 1 86 88 annotation1825 1 -35 range1825 1 21 26 annotation1833 1 HH117a mutation specific to I1020 and I1023 range1833 1 58 58 annotation1827 1 Complementary to Antisense mRNA I1023 range1827 1 56 90 annotation1830 1 LacO-1 region range1830 1 1 20 annotation1828 1 SsrA range1828 1 797 835 BBa_I1020_sequence 1 ataaatgtgagcggataacattgacattgtgagcggataacaagatactgagcacgccaaaaatcaataatcagacaacaagatgtgcagtactaagaaaaagcctttgactcaggaacaactcgaagatgcccgccgtctcaaggccatctacgagaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z