BBa_I1021
1
BBa_I1021
Antisense RNA w/BBa_I1020 Interference (KISS)
2003-01-31T12:00:00Z
2015-08-31T04:07:29Z
Anti-sense secondary structure adapted from: Mizuno, T., <em>Proc. Natl. Acad. Sci. USA</em> (1984) 81, 1966-1970<br>
Sequence for antisense RNA that interferes with BBa_I1020 mRNA using the KISS scheme.
false
false
_1_
0
24
7
It's complicated
false
<P> <P> <bb_part>BBa_I1021</bb_part> antisense RNA is designed to interfere with <bb_part>BBa_I1020</bb_part> mRNA with no expected secondary structure interactions (dubbed the KISS scheme for Keep is Simple Samantha). The sequence is designed to interfere exclusively with <bb_part>BBa_I1020</bb_part>, and act independently of <bb_part>BBa_I1010</bb_part> and <bb_part>BBa_I1011</bb_part>. These parts are designed to use codon space to code for the same protein (<bb_part>BBa_I1010</bb_part> and <bb_part>BBa_1020</bb_part> code for lambda cI) while allowing for independent anti-sense regulation of the <bb_part>BBa_I1010</bb_part> and <bb_part>BBa_1020</bb_part> transcripts via <bb_part>BBa_I1011</bb_part> and <bb_part>BBa_1021</bb_part><P>
false
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1837
1
start
range1837
1
71
73
annotation7047
1
BBa_I1021
range7047
1
1
104
annotation1836
1
suffix
range1836
1
101
104
annotation1839
1
cI CDS Reverse Complement
range1839
1
1
73
annotation1838
1
cI mRNA reverse complement
range1838
1
1
100
annotation1840
1
reverse complement
range1840
1
78
83
BBa_I1021_sequence
1
tctcgtagatggccttgagacggcgggcatcttcgagttgttcctgagtcaaaggctttttcttagtactcatcttgttgtctgattattgatttttcgctact
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z